Rabbit anti-Human C5 Polyclonal Antibody | anti-C5 antibody

C5, NT (Complement C5, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 4, Complement C5 beta Chain, Complement C5 alpha Chain, C5a Anaphylatoxin, Complement C5 alpha' Chain, CPAMD4) (Biotin)

Cat. Num. AAA6469960

• Gene Names: C5; C5D; C5a; C5b; ECLZB; CPAMD4
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: C5; Polyclonal Antibody; NT (Complement C5; C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 4; Complement C5 beta Chain; Complement C5 alpha Chain; C5a Anaphylatoxin; Complement C5 alpha' Chain; CPAMD4) (Biotin); anti-C5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C5.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-C5 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 253-281 in the N-terminal region of human C5.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) using C7850-24B. C5 (arrow) was detected using C7850-24B (2ug/ml))

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human breast carcinoma with C7850-24B, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of C7850-24B for Immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of MDA-231 cells using C7850-24B (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-C5 antibody

Antibodies; Complement

References

1. McClure, A., et.al., Rheumatology (Oxford) 48(11), 1369-1374 (2009). 2. DiScipio, R.G.et.al., J. Biol. Chem. 267(24), 17087-17094 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 38016947
• NCBI GeneID: 727
• NCBI Accession #: NP_001726.2
• NCBI GenBank Nucleotide #: NM_001735.2
• UniProt Accession #: P01031; Q14CJ0; Q27I61
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: complement C5 preproprotein
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: C5D; C5a; C5b; ECLZB; CPAMD4
• NCBI Protein Information: complement C5; prepro-C5; C5a anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• UniProt Protein Name: Complement C5
• Protein Family: C5a peptidase
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4
• UniProt Entry Name: CO5_HUMAN

NCBI Description

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq, Jul 2008]

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q33-q34

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; endopeptidase inhibitor activity; receptor binding; C5a anaphylatoxin chemotactic receptor binding

Biological Process: activation of MAPK activity; positive regulation of chemotaxis; in utero embryonic development; cytolysis; complement activation, alternative pathway; chemotaxis; glucose homeostasis; leukocyte migration during inflammatory response; complement activation; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of complement activation; response to stress; innate immune response; negative regulation of dopamine secretion; inflammatory response; complement activation, classical pathway

Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To