Sheep C5 Polyclonal Antibody | anti-C5 antibody

SHEEP ANTI HUMAN C5

Cat. Num. AAA220475

• Gene Names: C5; C5D; C5a; C5b; ECLZB; CPAMD4
• Applications: Immunodiffusion
• Synonyms: C5; Polyclonal Antibody; SHEEP ANTI HUMAN C5; anti-C5 antibody

• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Ig Fraction; Ig fraction - liquid
• Sequence Length: 1676

• Applicable Applications for anti-C5 antibody: Immunodiffusion (ID)
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 0.1% E-amino-n-caproic acid (EACA); 0.01% Benzamidine; 1 mM ethylenediaminetetraacetic acid (EDTA); Preparation
• Buffer Solution: Glycine buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-C5 antibody

Sheep anti human C5 polyclonal antibody recognizes complement component 5 (C5), a member of the complement system. The complement system consists of a tightly regulated network of proteins. Its activation leads to a sequential cascade of enzymatic reactions, resulting in opsonisation of the pathogens and their removal by phagocytes, as well as cell lysis. Three pathways have been described, the antibody-dependent pathway (classical pathway), the alternative pathway and the lectin activation pathway (Ehrnthaller et al. 2011, Nesargikar et al. 2012). The complement system plays a critical role in both innate and adaptive immune responses. It is also involved in tissue regeneration, tumor growth and some human pathologies (Sarma et al. 2011). Complement component 5 is the initiator of the effector terminal phase of the complement system. C5 convertase cleaves C5 into C5a, a potent anaphylatoxin, and C5b, ultimately resulting in the formation of the multimeric membrane attack complex (MAC) together with the other components of the terminal pathway (C5b-C9) (Noris et al. 2013). Sheep anti human polyclonal antibody gives a single arc when tested by immunoelectrophoresis (IEP) against human plasma. The identity has been confirmed by double diffusion against fresh human plasma and a known anti human C5.

NCBI and Uniprot Product Information

• NCBI GI #: 38016947
• NCBI GeneID: 727
• NCBI Accession #: NP_001726.2
• NCBI GenBank Nucleotide #: NM_001735.2
• UniProt Accession #: P01031; Q14CJ0; Q27I61
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: complement C5 preproprotein
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: C5D; C5a; C5b; ECLZB; CPAMD4
• NCBI Protein Information: complement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5a anaphylatoxin; anaphylatoxin C5a analog; prepro-C5
• UniProt Protein Name: Complement C5
• Protein Family: C5a peptidase
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4
• UniProt Entry Name: CO5_HUMAN

NCBI Description

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq, Jul 2008]

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q33-q34

Cellular Component: membrane attack complex; extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; endopeptidase inhibitor activity; C5a anaphylatoxin chemotactic receptor binding; receptor binding

Biological Process: in utero embryonic development; positive regulation of chemotaxis; activation of MAPK activity; cytolysis; complement activation, alternative pathway; chemotaxis; glucose homeostasis; leukocyte migration during inflammatory response; complement activation; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of complement activation; response to stress; innate immune response; negative regulation of dopamine secretion; inflammatory response; complement activation, classical pathway

Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To