Rabbit anti-Human C4BPA / C4BP Alpha Polyclonal Antibody | anti-C4BPA antibody

Anti-C4BPA / C4BP Alpha Antibody (aa470-499) IHC-plus

Cat. Num. AAA249505

• Gene Names: C4BPA; PRP; C4BP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A purified.
• Synonyms: C4BPA / C4BP Alpha; Polyclonal Antibody; Anti-C4BPA / C4BP Alpha Antibody (aa470-499) IHC-plus; Rabbit Polyclonal to Human C4BPA / C4BP Alpha; Human C4BPA / C4BP Alpha; C4BP; PrP; Proline-rich protein; anti-C4BPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This C4BPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-499 amino acids from the C-terminal region of human C4BPA.
• Purity/Purification: Protein A purified.
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 597

• Applicable Applications for anti-C4BPA antibody: Immunohistochemistry (IHC-P) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:10 - 1:50); IHC-P (1:100); WB (1:1000); Optimal dilutions/concentrations should be determined by the end user.
• Immunogen: C4BPA / C4BP Alpha antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human C4BPA.
• Immunogen Description: KLH-conjugated synthetic peptide from C-terminal region of human C4BPA.
• Immunogen Type: Synthetic peptide
• Target: C4BPA/C4BP Alpha
• Epitope: aa470-499
• Immunogen Species: Raised against human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months.; For long term storage store at -20°C.

Immunohistochemistry (IHC)

(Anti-C4BPA / C4BP antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(C4BPA Antibody western blot of WiDr cell line lysates (35 ug/lane). The C4BPA antibody detected the C4BPA protein (arrow).)

Flow Cytometry (FC/FACS)

(C4BPA Antibody flow cytometry of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-C4BPA antibody

C4BPA Antibody, C4BP Antibody, PrP Antibody, Proline-rich protein Antibody Description: C4BPA / C4BP Alpha is a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 4502503
• NCBI GeneID: 722
• NCBI Accession #: NP_000706.1
• NCBI GenBank Nucleotide #: NM_000715.3
• UniProt Accession #: P04003; Q5VVQ8
• NCBI Official Full Name: C4b-binding protein alpha chain
• NCBI Official Synonym Full Names: complement component 4 binding protein, alpha
• NCBI Official Symbol: C4BPA
• NCBI Official Synonym Symbols: PRP; C4BP
• NCBI Protein Information: C4b-binding protein alpha chain
• UniProt Protein Name: C4b-binding protein alpha chain
• Protein Family: C4b-binding protein
• UniProt Gene Name: C4BPA
• UniProt Synonym Gene Names: C4BP; C4bp; PRP
• UniProt Entry Name: C4BPA_HUMAN

NCBI Description

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

C4BPA: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: protein binding

Biological Process: complement activation, classical pathway; innate immune response; negative regulation of complement activation, classical pathway; positive regulation of protein catabolic process; regulation of complement activation