Goat anti-Mouse C3c Polyclonal Antibody | anti-c3c antibody

Goat anti Mouse C3c, conjugated with Biotin

Cat. Num. AAA573322

• Gene Names: c3c; C3-3; c3-2; unp301; zgc:103610
• Reactivity: Mouse
• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Dot Blot, Immunoblot, Western Blot
• Synonyms: C3c; Polyclonal Antibody; Goat anti Mouse C3c; conjugated with Biotin; anti-c3c antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to C3c fragment of Mouse complement factor C3

• Applicable Applications for anti-c3c antibody: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Dot Blot (DB), Immunoblotting (IB)
• Conjugation: Biotin
• Source Note: C3 is the most abundant complement protein in mouse serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways.
• Product: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
• Reactivity Note: The antiSerum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
• Preparation and Storage: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4 degree C--at least 10 years. Reconstituted at or below -20 degree C--3-5 years. Reconstituted at +4 degree C--7 days

Related Product Information for anti-c3c antibody

In immunoelectrophoresis against fresh mouse serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of mouse serum or plasma. The biotin immunoconjugate to mouse C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. In ELISA and Western blotting to identify mouse C3c in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:200; In ELISA and comparable nonprecipitating antibody-binding assays between 1:500 and 1:1,000.

Product Categories/Family for anti-c3c antibody

Complement; Coagulation; Haematology; Veterinary

NCBI and Uniprot Product Information

• NCBI GI #: 82524318
• NCBI GeneID: 565074
• NCBI Accession #: NP_001032313.1
• NCBI GenBank Nucleotide #: NM_001037236.1
• NCBI Official Full Name: complement component c3c
• NCBI Official Synonym Full Names: complement component c3c
• NCBI Official Symbol: c3c
• NCBI Official Synonym Symbols: C3-3; c3-2; unp301; zgc:103610
• NCBI Protein Information: complement component c3c; complement component C3-2