Goat anti-Human C3c Polyclonal Antibody | anti-c3c antibody

Goat anti Human C3c, conjugated with FITC

Cat. Num. AAA573278

• Gene Names: c3c; C3-3; c3-2; unp301; zgc:103610
• Reactivity: Human
• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
• Synonyms: C3c; Polyclonal Antibody; Goat anti Human C3c; conjugated with FITC; anti-c3c antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to C3c fragment of Human complement factor C3

• Applicable Applications for anti-c3c antibody: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
• Conjugation: FITC
• Product Type: Primary Antibodies
• Source: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Product: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
• Cross Reactivity: The antiSerum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
• Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in therapeutic procedures involving humans or animals.
• Preparation and Storage: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 degree C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4 degree C--at least 10 years. Reconstituted at or below -20 degree C--3-5 years. Reconstituted at +4 degree C--7 days

Related Product Information for anti-c3c antibody

Background:In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma. The fluorescent immunoconjugate to human C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the identification can be based on directly on laboratory findings. This immunoconjugate is not prediluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Product Categories/Family for anti-c3c antibody

Complement; Coagulation; Haematology

NCBI and Uniprot Product Information

• NCBI GI #: 82524318
• NCBI GeneID: 565074
• NCBI Accession #: NP_001032313.1
• NCBI GenBank Nucleotide #: NM_001037236.1
• NCBI Official Full Name: complement component c3c
• NCBI Official Synonym Full Names: complement component c3c
• NCBI Official Symbol: c3c
• NCBI Official Synonym Symbols: C3-3; c3-2; unp301; zgc:103610
• NCBI Protein Information: complement component c3c; complement component C3-2