Rabbit anti-Human C11orf53 Polyclonal Antibody | anti-C11orf53 antibody

C11orf53, CT (C11orf53, Uncharacterized protein C11orf53) (FITC)

Cat. Num. AAA6279465

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: C11orf53; Polyclonal Antibody; CT (C11orf53; Uncharacterized protein C11orf53) (FITC); Uncharacterized protein C11orf53; anti-C11orf53 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-C11orf53 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: C11orf53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the C-terminal region of human C11orf53.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(C11orf53 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the C11orf53 antibody detected the C11orf53 protein (arrow).)

Immunohistochemistry (IHC)

(C11orf53 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of C11orf53 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(C11orf53 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-C11orf53 antibody

Antibodies; KIAA; orf

References

Pittman, A.M., et al. Hum. Mol. Genet. 17(23):3720-3727(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 38348310
• NCBI GeneID: 341032
• NCBI Accession #: NP_940900.1
• NCBI GenBank Nucleotide #: NM_198498.1
• UniProt Accession #: Q8IXP5
• Molecular Weight: 25,393 Da
• NCBI Official Full Name: uncharacterized protein C11orf53
• NCBI Official Synonym Full Names: chromosome 11 open reading frame 53
• NCBI Official Symbol: C11orf53
• NCBI Protein Information: uncharacterized protein C11orf53
• UniProt Protein Name: Uncharacterized protein C11orf53
• UniProt Gene Name: C11orf53
• UniProt Entry Name: CK053_HUMAN