Western Blot (WB)
(Western blot analysis of lysates from Daudi, HL-60 cell line (from left to right), using C109B Antibody (C-term). MBS9212884 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit anti-Human C109B Polyclonal Antibody | anti-CCDC109B antibody
C109B Antibody (C-term)
Gene Names
CCDC109B; MCUb
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
C109B; Polyclonal Antibody; C109B Antibody (C-term); Calcium uniporter regulatory subunit MCUb; mitochondrial; MCUb; Coiled-coil domain-containing protein 109B; CCDC109B; MCUB; anti-CCDC109B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This C109B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-320 amino acids from the C-terminal region of human C109B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
291-320
Sequence Length
336
Applicable Applications for anti-CCDC109B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Negatively regulates the activity of MCU, the mitochondrial inner membrane calcium uniporter, and thereby modulates calcium uptake into the mitochondrion. Does not form functional calcium channels by itself. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.
Cellular Location
Mitochondrion inner membrane; Multi-passmembrane protein
Preparation and Storage
Maintain refrigerated at 2-8°C for up to weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from Daudi, HL-60 cell line (from left to right), using C109B Antibody (C-term). MBS9212884 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from Daudi, HL-60 cell line (from left to right), using C109B Antibody (C-term). MBS9212884 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(C109B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C109B antibody detected the C109B protein (arrow).)
Western Blot (WB)
(C109B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C109B antibody detected the C109B protein (arrow).)
Product Categories/Family for anti-CCDC109B antibody
References
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
calcium uniporter regulatory subunit MCUb, mitochondrial
NCBI Official Synonym Full Names
coiled-coil domain containing 109B
NCBI Official Symbol
CCDC109B
NCBI Official Synonym Symbols
MCUb
NCBI Protein Information
calcium uniporter regulatory subunit MCUb, mitochondrial
UniProt Protein Name
Calcium uniporter regulatory subunit MCUb, mitochondrial
UniProt Gene Name
CCDC109B
UniProt Synonym Gene Names
MCUB; MCUb
UniProt Entry Name
MCUB_HUMAN
Uniprot Description
CCDC109B: Belongs to the MCU family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 4q25
Cellular Component: mitochondrion; integral to mitochondrial inner membrane; intrinsic to membrane
Molecular Function: ion channel activity; calcium channel inhibitor activity
Biological Process: mitochondrial calcium ion homeostasis; mitochondrial calcium ion transport
Research Articles on CCDC109B
Similar Products
Product Notes
The CCDC109B ccdc109b (Catalog #AAA9212884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 291-320. The C109B Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C109B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the CCDC109B ccdc109b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C109B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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