Rabbit anti-Mouse, Rat C Peptide Polyclonal Antibody | anti-Ins2 antibody

C Peptide (mouse) polyclonal antibody

Cat. Num. AAA3013829

• Gene Names: Ins2; Mody; Ins-2; InsII; Mody4; AA986540
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: C Peptide; Polyclonal Antibody; C Peptide (mouse) polyclonal antibody; proinsulin precursor; Hyperproinsulinemia; INS; Insulin Precursor; IRDN; Proinsulin; Propeptide; C-Peptide; INS2_MOUSE; anti-Ins2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Specificity: C Peptide (mouse) polyclonal Antibody detects endogenous levels of C Peptide (mouse) protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-Ins2 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from mouse C Peptide:57-87/110
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C Peptide) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)

Related Product Information for anti-Ins2 antibody

C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

NCBI and Uniprot Product Information

• NCBI GI #: 297374794
• NCBI GeneID: 16334
• NCBI Accession #: NP_001172012.1
• NCBI GenBank Nucleotide #: NM_001185083.2
• UniProt Accession #: P01326
• Molecular Weight: 16.9 kDa
• NCBI Official Full Name: insulin-2 preproprotein
• NCBI Official Synonym Full Names: insulin II
• NCBI Official Symbol: Ins2
• NCBI Official Synonym Symbols: Mody; Ins-2; InsII; Mody4; AA986540
• NCBI Protein Information: insulin-2
• UniProt Protein Name: Insulin-2
• Protein Family: Insulin
• UniProt Gene Name: Ins2
• UniProt Synonym Gene Names: Ins-2

NCBI Description

This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.