Rabbit anti-Human c-Cbl Polyclonal Antibody | anti-CBL antibody

c-Cbl, phosphorylated (Tyr700) (E3 Ubiquitin-protein Ligase CBL, Casitas B-lineage Lymphoma Proto-oncogene, Proto-oncogene c-Cbl, RING Finger Protein 55, Signal Transduction Protein CBL, CBL, CBL2, RNF55)

Cat. Num. AAA628658

• Gene Names: CBL; CBL2; NSLL; C-CBL; RNF55
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: c-Cbl; Polyclonal Antibody; phosphorylated (Tyr700) (E3 Ubiquitin-protein Ligase CBL; Casitas B-lineage Lymphoma Proto-oncogene; Proto-oncogene c-Cbl; RING Finger Protein 55; Signal Transduction Protein CBL; CBL; CBL2; RNF55); Anti -c-Cbl; anti-CBL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human c-Cbl when phosphorylated at tyrosine 700.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-CBL antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry:1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human c-Cbl around the phosphorylation site of tyrosine 770 (T-E-YP-M-T).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CBL antibody

c-Cbl, product of c-cbl proto-oncogene, is a 120kD cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways. c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines. It has been demonstrated that tyrosines 700, 731, and 774 are the major tyrosine phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation.

Product Categories/Family for anti-CBL antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 29731
• NCBI GeneID: 867
• UniProt Accession #: P22681; A3KMP8
• Molecular Weight: 99,633 Da
• NCBI Official Full Name: c-cbl
• NCBI Official Synonym Full Names: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• NCBI Official Symbol: CBL
• NCBI Official Synonym Symbols: CBL2; NSLL; C-CBL; RNF55
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL; oncogene CBL2; OTTHUMP00000232087; proto-oncogene c-Cbl; RING finger protein 55; signal transduction protein CBL; casitas B-lineage lymphoma proto-oncogene
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: CBL
• UniProt Synonym Gene Names: CBL2; RNF55
• UniProt Entry Name: CBL_HUMAN

NCBI Description

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq]

Uniprot Description

CBL: an adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Participates in signal transduction in hematopoietic cells.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Adaptor/scaffold; EC 6.3.2.19; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; plasma membrane; flotillin complex; cytosol; nucleus

Molecular Function: protein binding; signal transducer activity; ephrin receptor binding; phosphotyrosine binding; zinc ion binding; ubiquitin-protein ligase activity; calcium ion binding; transcription factor activity; SH3 domain binding; ligase activity

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; positive regulation of receptor-mediated endocytosis; negative regulation of apoptosis

Disease: Noonan Syndrome-like Disorder With Or Without Juvenile Myelomonocytic Leukemia