Rabbit BUB1B Polyclonal Antibody | anti-BUB1B antibody

BUB1B (T368) Polyclonal Antibody

Cat. Num. AAA3002344

• Gene Names: BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BUB1B; Polyclonal Antibody; BUB1B (T368) Polyclonal Antibody; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L Protein SSK1; BUBR1; MAD3L; SSK1; anti-BUB1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BUB1B (T368) polyclonal antibody detects endogenous levels of BUB1B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1050

• Applicable Applications for anti-BUB1B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human BUB1B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BUB1B (T368) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264. 7 cell lysateLane3:PC12 cell lysate)

Immunoprecipitation (IP)

(Immunoprecipitation of HEK293T cell lysate using BUB1B (T368) polyclonal antibody (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using BUB1B (T368) # MBS3002344. “up-arrow” (supernatant) ;“down-arrow (deposition))

Western Blot (WB)

(Western Blot (WB) analysis of BUB1B (T368) pAb at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of BUB1B (T368) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:Jurkat whole cell lysate (40ug))

Related Product Information for anti-BUB1B antibody

Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 59814247
• NCBI GeneID: 701
• NCBI Accession #: NP_001202.4
• NCBI GenBank Nucleotide #: NP_001202.4
• UniProt Accession #: O60566
• Molecular Weight: ~ 130kDa
• NCBI Official Full Name: mitotic checkpoint serine/threonine-protein kinase BUB1 beta
• NCBI Official Synonym Full Names: BUB1 mitotic checkpoint serine/threonine kinase B
• NCBI Official Symbol: BUB1B
• NCBI Official Synonym Symbols: MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
• NCBI Protein Information: mitotic checkpoint serine/threonine-protein kinase BUB1 beta
• UniProt Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
• Protein Family: Mitotic checkpoint serine/threonine-protein kinase
• UniProt Gene Name: BUB1B
• UniProt Synonym Gene Names: BUBR1; MAD3L; SSK1; hBUBR1
• UniProt Entry Name: BUB1B_HUMAN

NCBI Description

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; BUB family

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: cell proliferation; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; apoptosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition

Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait