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Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTG3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Rabbit BTG3 Polyclonal Antibody | anti-BTG3 antibody

BTG3 Polyclonal Antibody

Gene Names
BTG3; ANA; TOB5; TOFA; TOB55
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
Applications
Immunohistochemistry
Purity
affinity purified by Protein A
Synonyms
BTG3; Polyclonal Antibody; BTG3 Polyclonal Antibody; Abundant in neuroepithelium area protein; ANA; BTG family member 3; Btg3; BTG3_HUMAN; Protein BTG3; Protein Tob5; anti-BTG3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
Clonality
Polyclonal
Specificity
BTG3 Polyclonal Antibody detects endogenous levels of BTG3 protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-BTG3 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-500
Immunogen
KLH conjugated synthetic peptide derived from human BTG3:1-100/252
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTG3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTG3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTG3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTG3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTG3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C,)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTG3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C,)
Related Product Information for anti-BTG3 antibody
The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008].

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
29,116 Da
NCBI Official Full Name
BTG family, member 3, isoform CRA_a
NCBI Official Synonym Full Names
BTG family, member 3
NCBI Official Symbol
BTG3
NCBI Official Synonym Symbols
ANA; TOB5; TOFA; TOB55
NCBI Protein Information
protein BTG3; protein Tob5; B-cell translocation gene 3; abundant in neuroepithelium area protein
UniProt Protein Name
Protein BTG3
Protein Family
UniProt Gene Name
BTG3
UniProt Synonym Gene Names
ANA; TOB5
UniProt Entry Name
BTG3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

BTG3: Overexpression impairs serum-induced cell cycle progression from the G0/G1 to S phase. Belongs to the BTG family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; negative regulation of mitotic cell cycle

Research Articles on BTG3

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Product Notes

The BTG3 btg3 (Catalog #AAA3015520) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BTG3 Polyclonal Antibody reacts with Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's BTG3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-500. Researchers should empirically determine the suitability of the BTG3 btg3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTG3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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