Rabbit anti-Human BRSK1 Polyclonal Antibody | anti-BRSK1 antibody

BRSK1, NT (BR Serine/Threonine-protein Kinase 1, SAD1 Kinase, Serine/Threonine-protein Kinase SAD-B, KIAA1811, SAD1) (Azide free) (HRP)

Cat. Num. AAA6465746

• Gene Names: BRSK1; hSAD1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: BRSK1; Polyclonal Antibody; NT (BR Serine/Threonine-protein Kinase 1; SAD1 Kinase; Serine/Threonine-protein Kinase SAD-B; KIAA1811; SAD1) (Azide free) (HRP); anti-BRSK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BRSK1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BRSK1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human BRSK1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-KIAA181 Pab in mouse liver tissue lysate (35ug/lane). KIAA181(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-BRSK1 antibody

Antibodies; Protein Kinases

References

1. Lu, R., et al., J. Biol. Chem. 279(30):31164-31170 (2004). 2. Lizcano, J.M., et al., EMBO J. 23(4):833-843 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 24308326
• NCBI GeneID: 84446
• NCBI Accession #: NP_115806
• NCBI GenBank Nucleotide #: NM_032430.1
• UniProt Accession #: Q8TDC3; Q5J5B5; Q8NDD0; Q8NDR4; Q8TDC2; Q96AV4; Q96JL4; F1DG44
• Molecular Weight: 38,782 Da
• NCBI Official Full Name: serine/threonine-protein kinase BRSK1
• NCBI Official Synonym Full Names: BR serine/threonine kinase 1
• NCBI Official Symbol: BRSK1
• NCBI Official Synonym Symbols: hSAD1
• NCBI Protein Information: serine/threonine-protein kinase BRSK1
• UniProt Protein Name: Serine/threonine-protein kinase BRSK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: BRSK1
• UniProt Synonym Gene Names: KIAA1811; SAD1; SADB; BR serine/threonine-protein kinase 1; SAD1 homolog; hSAD1
• UniProt Entry Name: BRSK1_HUMAN

Uniprot Description

BRSK1: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. Acts as checkpoint kinase upon DNA damage induced by UV or methyl methane sulfonate. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. Translocates from the cytoplasm to the nucleus in response to UV- or MMS-induced DNA damage. BRSK1 and BRSK2 are required for neuronal polarization. Widely expressed with highest levels in the brain and testis. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; EC 2.7.11.1; Protein kinase, CAMK; Kinase, protein; CAMK group; CAMKL family; BRSK subfamily

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; centrosome; synaptic vesicle; cytoplasm; cell junction; nucleus

Molecular Function: protein serine/threonine kinase activity; gamma-tubulin binding; tau-protein kinase activity; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: neuron differentiation; axonogenesis; establishment of cell polarity; neurotransmitter secretion; response to ionizing radiation; G2/M transition DNA damage checkpoint; centrosome duplication; response to DNA damage stimulus; protein amino acid phosphorylation; response to UV