Western Blot (WB)
(Western Blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2)
Rabbit BRG1 Polyclonal Antibody | anti-BRG1 antibody
BRG1 Polyclonal Antibody
Gene Names
SMARCA4; BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
BRG1; Polyclonal Antibody; BRG1 Polyclonal Antibody; BAF190A; SMARCA4; ATP dependent helicase SMARCA4; ATP-dependent helicase SMARCA4; BAF 190; BAF190; Brahma protein like 1; BRG1 associated factor 190A; BRG1 protein; BRG1-associated factor 190A; BRM/SWI2 related gene 1; Global transcription activator homologous sequence; global transcription activator snf2l4; Homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; MRD16; Nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; Protein BRG1; RTPS2; SMARC A4; SMCA4; SNF2; SNF2 beta; SNF2 like 4; SNF2-beta; SNF2B; SNF2L4; SNF2LB; Sucrose nonfermenting like 4; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; SWI2; Transcription activator BRG1; anti-BRG1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BRG1 polyclonal antibody detects endogenous levels of BRG1 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1647
Applicable Applications for anti-BRG1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2)
Western Blot (WB)
(Western Blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2)
Immunocytochemistry (ICC)
(ICC staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-BRG1 antibody
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2alpha) and Brg-1 (also designated SNF2beta) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
185kDa
NCBI Official Full Name
transcription activator BRG1 isoform B
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
NCBI Official Symbol
SMARCA4
NCBI Official Synonym Symbols
BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta
NCBI Protein Information
transcription activator BRG1
UniProt Protein Name
Transcription activator BRG1
Protein Family
UniProt Gene Name
SMARCA4
UniProt Synonym Gene Names
BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
UniProt Entry Name
SMCA4_HUMAN
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Research Articles on BRG1
Similar Products
Product Notes
The BRG1 smarca4 (Catalog #AAA3012321) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRG1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1,000-1:5,000 ICC: 1:50-1:200 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the BRG1 smarca4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
