Rabbit BRF2 Polyclonal Antibody | anti-BRF2 antibody
BRF2 Antibody
Gene Names
BRF2; BRFU; TFIIIB50
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
BRF2; Polyclonal Antibody; BRF2 Antibody; B-related factor 2; BRF-2; BRF2_HUMAN; BRFU; FLJ11052; hBRFU; hTFIIIB50; RNA polymerase III transcription initiation factor BRF2; TFIIIB50; Transcription factor IIB- related factor TFIIIB50; Transcription factor IIIB 50 kDa subunit; anti-BRF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
BRF2 Antibody detects endogenous levels of total BRF2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BRF2 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
Belongs to the TFIIB family.
Post Translational Modifications
In response to oxidative stress, Cys-361 is reversibly oxidized to cysteine sulfenic acid. Oxidation of Cys-361 impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress.
Subunit Structure
Component of TFIIIB complexes. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. Interacts with TBP; this interaction promotes recruitment of BRF2 to TATA box-containing promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.
Function
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,104 Da
NCBI Official Full Name
transcription factor IIIB 50 kDa subunit
NCBI Official Synonym Full Names
BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit
NCBI Official Symbol
BRF2
NCBI Official Synonym Symbols
BRFU; TFIIIB50
NCBI Protein Information
transcription factor IIIB 50 kDa subunit; B-related factor 2; BRF-2; BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like; RNA polymerase III transcription initiation factor BRFU; hBRFU; hTFIIIB50; transcription factor IIB- relat
UniProt Protein Name
Transcription factor IIIB 50 kDa subunit
Protein Family
UniProt Gene Name
BRF2
UniProt Synonym Gene Names
BRFU; TFIIIB50; hTFIIIB50; BRF-2
UniProt Entry Name
BRF2_HUMAN
Similar Products
Product Notes
The BRF2 brf2 (Catalog #AAA9629213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRF2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the BRF2 brf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
