Rabbit anti-Human BRF1 Polyclonal Antibody | anti-BRF1 antibody

BRF1 (BRF, GTF3B, TAF3B2, TAF3C, Transcription Factor IIIB 90kD Subunit, B-related Factor 1, TATA Box-binding Protein-associated Factor, RNA Polymerase III, Subunit 2, MGC105048) (PE)

Cat. Num. AAA6371441

• Gene Names: BRF1; BRF; CFDS; hBRF; BRF-1; GTF3B; TAF3C; TAF3B2; TF3B90; TAFIII90; TFIIIB90; HEL-S-76p
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BRF1; Polyclonal Antibody; BRF1 (BRF; GTF3B; TAF3B2; TAF3C; Transcription Factor IIIB 90kD Subunit; B-related Factor 1; TATA Box-binding Protein-associated Factor; RNA Polymerase III; Subunit 2; MGC105048) (PE); anti-BRF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BRF1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 473

• Applicable Applications for anti-BRF1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human BRF1, aa1-473 (NP_663718.1).
• Immunogen Sequence: MTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEEECDPPSYTAGQRKLRMKQLEQVLSKKLEEVEGEISSYQDAIEIELENSRPKAKGGLASLAKDGSTEDTASSLCGEEDTEDEELEAAASHLNKDLYRELLGGAPGSSEAAGSPEWGGRPPALGSLLDPLPTAASLGISDSIRECISSQSSDPKDASGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKEARIAKEKELGIYKEHKPKKSCKRREPIQASTAREAIEKMLEQKKISSKINYSVLRGLSSAGGGSPHREDAQPEHSASARKLSRRRTPASRSGADPVTSVGKRLRPLVSTQPAKKVATGEALLPSSPTLGAEPARPQAVLVESGPVSYHADEEADEEEPDEEDGEPCVSALQMMGSNDYGCDGDEDDGY
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of BRF1 expression in transfected 293T cell line by BRF1 polyclonal antibody. Lane 1: BRF1 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-BRF1 antibody

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Two alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. Other transcript variants are possible, but their full-length natures have not been completely characterized. [provided by RefSeq].

Product Categories/Family for anti-BRF1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 22035558
• NCBI GeneID: 2972
• NCBI Accession #: NP_663718.1
• NCBI GenBank Nucleotide #: NM_145685.1
• UniProt Accession #: Q92994
• NCBI Official Full Name: transcription factor IIIB 90 kDa subunit isoform 3
• NCBI Official Synonym Full Names: BRF1 RNA polymerase III transcription initiation factor subunit
• NCBI Official Symbol: BRF1
• NCBI Official Synonym Symbols: BRF; CFDS; hBRF; BRF-1; GTF3B; TAF3C; TAF3B2; TF3B90; TAFIII90; TFIIIB90; HEL-S-76p
• NCBI Protein Information: transcription factor IIIB 90 kDa subunit
• UniProt Protein Name: Transcription factor IIIB 90 kDa subunit
• Protein Family: Transcription factor
• UniProt Gene Name: BRF1
• UniProt Synonym Gene Names: BRF; GTF3B; TAF3B2; TAF3C; TFIIIB90; hTFIIIB90; BRF-1; hBRF
• UniProt Entry Name: TF3B_HUMAN

NCBI Description

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]

Uniprot Description

GTF3B: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. Belongs to the TFIIB family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 14q

Cellular Component: nucleoplasm; transcription factor TFIIIB complex

Molecular Function: zinc ion binding; TATA-binding protein binding

Biological Process: transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; tRNA transcription; regulation of transcription, DNA-dependent; gene expression; regulation of mRNA stability; rRNA transcription

Disease: Cerebellofaciodental Syndrome