Rabbit anti-Mouse, Rat BRCC36 Polyclonal Antibody | anti-BRCC36 antibody

RABBIT ANTI BRCC36

Cat. Num. AAA222935

• Gene Names: BRCC3; C6.1A; BRCC36; CXorf53
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: BRCC36; Polyclonal Antibody; RABBIT ANTI BRCC36; anti-BRCC36 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human BRCA1/BRCA2 complex subunit 36 (BRCC36), a 36.7kDa nuclear protein. ; ; BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity. ; ; Studies have demonstrated that BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation. ; ; Research has shown that BRCC36 is aberrantly expressed in the majority of breast tumours indicating a potential role in the pathogenesis of the disease. It has been hypothesized that BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumours.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 291

• Applicable Applications for anti-BRCC36 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 36kDa in MCF7 cell lysates.; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: An 18 amino acid peptide from near the amino terminus of human BRCC36.; Buffer Solution
• Antiserum Preparation: Antiserum to human BRCC36 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from MCF7 human breast adenocarcinoma cells probed with Rabbit anti Human BRCC36 (MBS222935) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Formalin fixed, paraffin embedded human brain stained with Rabbit anti Human BRCC36 (MBS222935) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-BRCC36 antibody

MBS222915 recognises human BRCA1/BRCA2 complex subunit 36 (BRCC36), a 36.7kDa nuclear protein. BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity. Studies have demonstrated that BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation. Research has shown that BRCC36 is aberrantly expressed in the majority of breast tumours indicating a potential role in the pathogenesis of the disease. It has been hypothesized that BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumours.

NCBI and Uniprot Product Information

• NCBI GI #: 64762484
• NCBI GeneID: 79184
• NCBI Accession #: NP_001018065.1
• NCBI GenBank Nucleotide #: NM_001018055.2
• Molecular Weight: 28,027 Da
• NCBI Official Full Name: lys-63-specific deubiquitinase BRCC36 isoform 2
• NCBI Official Synonym Full Names: BRCA1/BRCA2-containing complex, subunit 3
• NCBI Official Symbol: BRCC3
• NCBI Official Synonym Symbols: C6.1A; BRCC36; CXorf53
• NCBI Protein Information: lys-63-specific deubiquitinase BRCC36; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36
• UniProt Protein Name: Lys-63-specific deubiquitinase BRCC36
• UniProt Gene Name: BRCC3
• UniProt Synonym Gene Names: BRCC36; C6.1A; CXorf53
• UniProt Entry Name: BRCC3_HUMAN

NCBI Description

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]

Uniprot Description

BRCC3: Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA. Belongs to the peptidase M67A family. BRCC36 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.-; Ubiquitin conjugating system; Ubiquitin-specific protease; Oncoprotein

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; nuclear ubiquitin ligase complex; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; metallopeptidase activity; metal ion binding; ubiquitin-specific protease activity; polyubiquitin binding; enzyme regulator activity

Biological Process: positive regulation of DNA repair; regulation of catalytic activity; double-strand break repair; response to ionizing radiation; proteolysis; G2/M transition DNA damage checkpoint; response to X-ray