Rabbit BRCA1 Polyclonal Antibody | anti-BRCA1 antibody

Anti-BRCA1 antibody

Cat. Num. AAA176954

• Gene Names: BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: BRCA1; Polyclonal Antibody; Anti-BRCA1 antibody; Breast cancer type 1 susceptibility protein; breast cancer 1; early onset; BRAC 1 antibody; BRCA 1 antibody; BRCA1 antibody; BRCA1/BRCA2 containing complex subunit 1 antibody; BRCA1/BRCA2-containing complex; subunit 1 antibody; BRCA1_HUMAN antibody; BRCAI antibody; BRCC 1 antibody; BRCC1 antibody; Breast and ovarian cancer susceptibility protein 1 antibody; Breast Cancer 1 antibody; Breast Cancer 1 Early Onset antibody; Breast cancer type 1 susceptibility protein antibody; Breast Ovarian Cancer Susceptibility antibody; BROVCA1 antibody; IRIS antibody; Papillary Serous Carcinoma Of The Peritoneum antibody; PNCA4 antibody; PPP1R53 antibody; Protein phosphatase 1 regulatory subunit 53 antibody; PSCP antibody; RING finger protein 53 antibody; RNF53 antibody; anti-BRCA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 1863

• Applicable Applications for anti-BRCA1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: E Coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-BRCA1 Picoband antibody, MBS176954-1.JPGIHC(P): Mouse Testis Tissue )

Immunohistochemistry (IHC)

(Anti-BRCA1 Picoband antibody, MBS176954-2.JPGIHC(P): Rat Testis Tissue )

Immunohistochemistry (IHC)

(Anti-BRCA1 Picoband antibody, MBS176954-3.JPGIHC(P): Human Mammary Cancer Tissue )

Testing Data

(Anti-BRCA1 Picoband antibody, MBS176954-4.jpgAll lanes: Anti-BRCA1(MBS176954) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugPredicted bind size: 207KDObserved bind size: 207KD )

Related Product Information for anti-BRCA1 antibody

Description: Rabbit IgG polyclonal antibody for Breast cancer type 1 susceptibility protein(BRCA1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.

NCBI and Uniprot Product Information

• NCBI GI #: 728984
• NCBI GeneID: 672
• UniProt Accession #: P38398; O15129; Q1RMC1; Q3LRJ0; Q3LRJ6; Q6IN79; Q7KYU9
• Molecular Weight: 210,266 Da
• NCBI Official Full Name: Breast cancer type 1 susceptibility protein
• NCBI Official Synonym Full Names: breast cancer 1, early onset
• NCBI Official Symbol: BRCA1
• NCBI Official Synonym Symbols: IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
• NCBI Protein Information: breast cancer type 1 susceptibility protein; RING finger protein 53; BRCA1/BRCA2-containing complex, subunit 1; protein phosphatase 1, regulatory subunit 53; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1
• UniProt Protein Name: Breast cancer type 1 susceptibility protein
• Protein Family: BRCA1-A complex
• UniProt Gene Name: BRCA1
• UniProt Synonym Gene Names: RNF53
• UniProt Entry Name: BRCA1_HUMAN

NCBI Description

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Uniprot Description

BRCA1: the BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACC1 and preventing its dephosphorylation. Defects in BRCA1 are a cause of genetic susceptibility to breast cancer. Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association may be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts (via BRCT domains) with CTIP. Associates with RNA polymerase II holoenzyme. Interacts with SMC1 and COBRA1. Interacts (via BRCT domains) with BRIP1. Interacts with FANCD2 (ubiquitinated). Interacts with BAP1. Interacts with Artemis and claspin. Interacts with H2AFX (phosphorylated on S140). Interacts with CHK1. Interacts with BRCC3. Five human isoforms are produced by alternative splicing and alternative initiation. Isoform 1 and isoform 3 are widely expressed. Isoform 1 is largely nuclear, while isoforms 3 and 5 are cytoplasmic.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Tumor suppressor; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; gamma-tubulin ring complex; protein complex; condensed nuclear chromosome; cytoplasm; BRCA1-BARD1 complex; plasma membrane; chromosome; nucleus; ribonucleoprotein complex; ubiquitin ligase complex

Molecular Function: tubulin binding; protein binding; enzyme binding; androgen receptor binding; DNA binding; zinc ion binding; RNA binding; ubiquitin protein ligase binding; transcription coactivator activity; damaged DNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: genetic imprinting; apoptosis; positive regulation of transcription, DNA-dependent; dosage compensation, by inactivation of X chromosome; centrosome cycle; protein ubiquitination; negative regulation of histone H3-K4 methylation; positive regulation of histone H3-K4 methylation; negative regulation of histone acetylation; chromosome segregation; regulation of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; double-strand break repair; DNA replication; fatty acid biosynthetic process; negative regulation of fatty acid biosynthetic process; positive regulation of histone H3-K9 methylation; negative regulation of histone H3-K9 methylation; protein autoubiquitination; chordate embryonic development; positive regulation of DNA repair; postreplication repair; DNA repair; negative regulation of centriole replication; double-strand break repair via homologous recombination; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; response to estrogen stimulus; regulation of transcription from RNA polymerase III promoter; positive regulation of protein ubiquitination; androgen receptor signaling pathway; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; response to ionizing radiation; negative regulation of transcription, DNA-dependent; G2/M transition DNA damage checkpoint; response to DNA damage stimulus

Disease: Breast-ovarian Cancer, Familial, Susceptibility To, 1; Pancreatic Cancer, Susceptibility To, 4; Breast Cancer