Rabbit botF Polyclonal Antibody | anti-botF antibody

botF Antibody

Cat. Num. AAA7113778

• Reactivity: Clostridium botulinum
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G Purified
• Synonyms: botF; Polyclonal Antibody; botF Antibody; Botulinum neurotoxin type F (BoNT/F) (EC 3.4.24.69) (Bontoxilysin-F) [Cleaved into: Botulinum neurotoxin F light chain; Botulinum neurotoxin F heavy chain]; anti-botF antibody

• Host: Rabbit
• Reactivity: Clostridium botulinum
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G Purified
• Form/Format: Liquid
• Sequence Length: 1274

• Applicable Applications for anti-botF antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:5000
• Immunogen: Recombinant Clostridium botulinum Botulinum neurotoxin type F protein (1-436AA)
• Conjugate: Non-Conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Species: Clostridium botulinum
• Target Names: botF
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in Recombinant proteinAll lanes: botF antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60 kDaObserved band size: 60 kDa)

Related Product Information for anti-botF antibody

Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '58-Gln-|-Lys-59' bond of synaptobrevins-1 and -2.

NCBI and Uniprot Product Information

• NCBI GI #: 399137
• UniProt Accession #: P30996
• NCBI Official Full Name: Botulinum neurotoxin type F
• UniProt Protein Name: Botulinum neurotoxin type F
• Protein Family: Botulinum neurotoxin
• UniProt Gene Name: botF
• UniProt Synonym Gene Names: LC; HC

Uniprot Description

Botulinum neurotoxin type F: Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin F which may have 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release (). Whole toxin only has protease activity after reduction, which releases LC (PubMed:8505288). Requires complex eukaryotic host polysialogangliosides for full neurotoxicity (). It is not clear whether a synaptic vesicle protein acts as its receptor; there is evidence for and against SV2 fulfilling this function ().