Rabbit BNIP3 Polyclonal Antibody | anti-BNIP3 antibody

BNIP3 Polyclonal Antibody

Cat. Num. AAA191307

• Gene Names: BNIP3; NIP3
• Applications: Immunoblot, Western Blot
• Synonyms: BNIP3; Polyclonal Antibody; BNIP3 Polyclonal Antibody; anti-BNIP3 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody recognizes human and mouse BNIP3.
• Form/Format: 100ug (1mg/ml) peptide affinity-purified antibody in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
• Sequence Length: 259

• Applicable Applications for anti-BNIP3 antibody: Immunoblot
• Application Notes: Immunoblot: 1ug/ml. A band of ~30kDa is detected; an additional ~60kDa band, complexed BNIP3, may also be detected.; Dilute in PBS or medium which is identical to that used in the assay system
• Immunogen: Synthetic peptide corresponding to amino acids at the C-terminus of human BNIP3.
• Preparation and Storage: This antibody is stable for at least one (1) year at-20 degree C.

Testing Data

(Detection of BNIP3 in HeLa (left) and 293T (right) cell lysates at 1ug/ml)

Testing Data

(Detection of BNIP3 and complexed BNIP3 in mouse kidney lysate at 1ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence: use at 10ug/ml. Detection of BNIP3 in formalin-fixed NIH 3T3 cells at 10ug/ml. DAPI (blue) nuclear stain, Texas red actin stain, and FITC (green) BNIP3 stain.)

Related Product Information for anti-BNIP3 antibody

BNIP3 is a member of the apoptotic Bcl-2 protein family that is involved in both necrosis and apoptosis. Humans and other animals, as well as lower eukaryotes, encode several BNIP3 paralogues including human BNIP3L which induces apoptosis by interacting with viral and cellular anti-apoptosis proteins. BNIP3 interacts with the E1B 19kDa protein, which is responsible for the protection of virally induced cell death, as well as with E1B-like sequences of BCL2 which is also an apoptotic protector. The BNIP3 gene contains a BH3 domain and a transmembrane domain that have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by the BNIP3 gene is known to induce apoptosis, even in the presence of BCL2.

NCBI and Uniprot Product Information

• NCBI GI #: 734703979
• NCBI GeneID: 664
• NCBI Accession #: NP_004043.3
• NCBI GenBank Nucleotide #: NM_004052.3
• UniProt Accession #: Q12983; O14620; Q96GP0
• Molecular Weight: 27,832 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• NCBI Official Synonym Full Names: BCL2 interacting protein 3
• NCBI Official Symbol: BNIP3
• NCBI Official Synonym Symbols: NIP3
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Protein Family: BNIP3
• UniProt Gene Name: BNIP3
• UniProt Synonym Gene Names: NIP3

NCBI Description

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

Uniprot Description

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.