Rabbit anti-Human, Mouse BMPR2 Polyclonal Antibody | anti-BMPR2 antibody

BMPR2, NT (Bone morphogenetic protein receptor type-2, Bone morphogenetic protein receptor type II, BMP type II receptor, BMPR-II, PPH1) (MaxLight 490)

Cat. Num. AAA6278676

• Gene Names: BMPR2; BMR2; PPH1; BMPR3; BRK-3; T-ALK; BMPR-II
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: BMPR2; Polyclonal Antibody; NT (Bone morphogenetic protein receptor type-2; Bone morphogenetic protein receptor type II; BMP type II receptor; BMPR-II; PPH1) (MaxLight 490); Bone morphogenetic protein receptor type-2; PPH1; anti-BMPR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BMPR2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-BMPR2 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 27-56 in the N-terminal region of human BMPR2, UniProt Accession #Q13873.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted withMBS6012256, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using MBS6012256 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BMPR2 antibody

MaxLight490 is a new Blue-Green photostable dye conjugate comparable to DyLight488, Alexa Fluor488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Product Categories/Family for anti-BMPR2 antibody

Antibodies; Receptors; Growth Factor

References

1. Pouliot, F., et al., Cancer Res. 63(2):277-281 (2003). 2. Nishihara, A., et al., Mol. Biol. Cell 13 (9):3055-3063 (2002). 3. Humbert, M., et al., Eur Respir J 20(3):518-523 (2002). 4. Vitt, U.A., et al., Biol. Reprod. 67(2):473-480 (2002). 5. Nohe, A., et al., J. Biol. Chem. 277(7):5330-5338 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 15451916
• NCBI GeneID: 659
• NCBI Accession #: NP_001195.2
• NCBI GenBank Nucleotide #: NM_001204.6
• UniProt Accession #: Q13873; Q16569; Q4ZG08; Q53SA5; Q585T8
• Molecular Weight: 115,201 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-2
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type II (serine/threonine kinase)
• NCBI Official Symbol: BMPR2
• NCBI Official Synonym Symbols: BMR2; PPH1; BMPR3; BRK-3; T-ALK; BMPR-II
• NCBI Protein Information: bone morphogenetic protein receptor type-2; BMPR-2; BMP type-2 receptor; BMP type II receptor; type II activin receptor-like kinase; bone morphogenetic protein receptor type II; type II receptor for bone morphogenetic protein-4
• UniProt Protein Name: Bone morphogenetic protein receptor type-2
• Protein Family: Bone morphogenetic protein receptor
• UniProt Gene Name: BMPR2
• UniProt Synonym Gene Names: PPH1; BMP type-2 receptor; BMPR-2; BMP type II receptor; BMPR-II
• UniProt Entry Name: BMPR2_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease. [provided by RefSeq, Jul 2008]

Uniprot Description

BMPR2: a serine/threonine-protein kinase receptor for bone morphogenetic protein (BMP). Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1), a weakly penetrant dominant disorder, associated with lesions in pulmonary arterioles, elevated pulmonary arterial pressure, and right ventricular failure.

Protein type: EC 2.7.11.30; Kinase, protein; Cell cycle regulation; Protein kinase, TKL; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: extracellular space; cell surface; cell soma; integral to plasma membrane; apical plasma membrane; dendrite; cytoplasm; plasma membrane; basal plasma membrane; caveola

Molecular Function: transforming growth factor beta receptor activity; protein binding; growth factor binding; metal ion binding; activin receptor activity, type II; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: limb development; transcription from RNA polymerase II promoter; regulation of lung blood pressure; activin receptor signaling pathway; protein amino acid phosphorylation; anterior/posterior pattern formation; BMP signaling pathway; proteoglycan biosynthetic process; lymphangiogenesis; transmembrane receptor protein serine/threonine kinase signaling pathway; positive regulation of BMP signaling pathway; negative regulation of systemic arterial blood pressure; chondrocyte development; positive regulation of bone mineralization; cellular response to starvation; negative regulation of vasoconstriction; regulation of cell proliferation; positive regulation of osteoblast differentiation; mesoderm formation; positive regulation of axon extension involved in axon guidance; positive regulation of endothelial cell proliferation; blood vessel remodeling; brain development; negative regulation of cell growth; vascular endothelial growth factor receptor signaling pathway; alveolus development

Disease: Pulmonary Venoocclusive Disease 1, Autosomal Dominant; Pulmonary Hypertension, Primary, 1