Testing Data #1
(Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (MBS221841))
Rabbit BMP-2 Polyclonal Antibody | anti-BMP-2 antibody
RABBIT ANTI HUMAN BMP-2
Gene Names
BMP2; BDA2; BMP2A
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
BMP-2; Polyclonal Antibody; RABBIT ANTI HUMAN BMP-2; anti-BMP-2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
396
Applicable Applications for anti-BMP-2 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
Application Notes
ELISA: This purified human BMP-2antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human BMP-2 antibody as the detection reagent.
Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions.
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 5.0ug/ml
Immunohistology - Paraffin: Maximum Dilution: 0.25ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 1.0ug/ml
Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions.
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 5.0ug/ml
Immunohistology - Paraffin: Maximum Dilution: 0.25ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 1.0ug/ml
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Antiserum Preparation
Antisera to human BMP-2 were raised by repeated immunisations of rabbits with highly purified antigen.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (MBS221841))
Testing Data #1
(Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (MBS221841))
Testing Data #2
(Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (MBS221841))
Testing Data #2
(Staining of formalin fixed, paraffin embedded human colo-rectal adenocarcinoma with Rabbit anti Human BMP-2 (MBS221841))
Testing Data #3
(U-2 osteosarcoma cells stained with Rabbit anti Human BMP-2 (MBS221841) green, tubulin, red and nuclei, blue)
Testing Data #3
(U-2 osteosarcoma cells stained with Rabbit anti Human BMP-2 (MBS221841) green, tubulin, red and nuclei, blue)
Related Product Information for anti-BMP-2 antibody
Rabbit anti Human BMP-2 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2 (BMP-2/BMP-2a), full-length 396 amino acids. BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis. BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation. The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,702 Da
NCBI Official Full Name
bone morphogenetic protein 2 preproprotein
NCBI Official Synonym Full Names
bone morphogenetic protein 2
NCBI Official Symbol
BMP2
NCBI Official Synonym Symbols
BDA2; BMP2A
NCBI Protein Information
bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A
UniProt Protein Name
Bone morphogenetic protein 2
Protein Family
UniProt Gene Name
BMP2
UniProt Synonym Gene Names
BMP2A; BMP-2; BMP-2A
UniProt Entry Name
BMP2_HUMAN
NCBI Description
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]
Uniprot Description
Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).
Research Articles on BMP-2
Similar Products
Product Notes
The BMP-2 bmp2 (Catalog #AAA221841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BMP-2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB). ELISA: This purified human BMP-2antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human BMP-2 antibody as the detection reagent. Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions. ELISA: Minimum Dilution: 0.5; Maximum Dilution: 5.0ug/ml Immunohistology - Paraffin: Maximum Dilution: 0.25ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 1.0ug/ml. Researchers should empirically determine the suitability of the BMP-2 bmp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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