Rabbit anti-Human BLVRB Polyclonal Antibody | anti-BLVRB antibody

Anti-BLVRB Antibody, Rabbit Polyclonal

Cat. Num. AAA8106787

• Gene Names: BLVRB; FLR; BVRB; SDR43U1; HEL-S-10
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: BLVRB; Polyclonal Antibody; Anti-BLVRB Antibody; Rabbit Polyclonal; BLVRB/biliverdin reductase B Antibody; Rabbit PAb; Antigen Affinity Purified; biliverdin reductase B; Anti-BVRB Antibody; Anti-FLR Antibody; Anti-HEL-S-10 Antibody; Anti-SDR43U1 Antibody; anti-BLVRB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human BLVRB/biliverdin reductase B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BLVRB antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human BLVRB/biliverdin reductase B protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human BLVRB/biliverdin reductase B (rh BLVRB/biliverdin reductase B). BLVRB/biliverdin reductase B specific IgG was purified by Human BLVRB/biliverdin reductase B affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(BLVRB was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Caco-2 Whole Cell Lysate2 uL anti-BLVRB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BLVRB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)

Western Blot (WB)

(Anti-BLVRB rabbit polyclonal antibody at 1:500 dilutionLane A: Caco-2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa)

Western Blot (WB)

(Anti-BLVRB rabbit polyclonal antibody at 1:500 dilution Lane A: BLVRB konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:22 kDa)

Related Product Information for anti-BLVRB antibody

Biliverdin reductase (hBVR) is a serine/threonine kinase that catalyzes reduction of the heme oxygenase (HO) activity product, biliverdin, to bilirubin. BVR consists of an N-terminal dinucleotide-binding domain (Rossmann-fold) and a C-terminal domain that contains a six-stranded beta-sheet that is flanked on one face by several alpha-helices. The C-terminal and N-terminal domains interact extensively, forming the active site cleft at their interface. Biliverdin reductase (BVR) catalyzes the last step in heme degradation by reducing the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a beta-nicotinamide adenine dinucleotide (NADH) or beta-nicotinamide adenine dinucleotide phosphate (NADPH) cofactor. It is now recognized that human BVR (hBVR) is a dual-specificity kinase (Ser/Thr and Tyr) upstream activator of the insulin/insulin growth factor-1 (IGF-1) and mitogen-activated protein kinase (MAPK) signaling pathways. Human BVR (hBVR) is essential for MAPK-extracellular signal-regulated kinase (ERK)1/2 (MEK)-eukaryotic-like protein kinase (Elk) signaling and has been identified as the cytoplasm-nuclear heme transporter of ERK1/2 and hematin, the key components of stress-responsive gene expression.

References

Kapitulnik J, et al. (2009) Pleiotropic functions of biliverdin reductase: cellular signaling and generation of cytoprotective and cytotoxic bilirubin. Trends in Pharmacological Sciences. 30(3): 129-37.Ahmad Z, et al. (2002) Human Biliverdin Reductase Is a Leucine Zipper-like DNA-binding Protein and Functions in Transcriptional Activation of Heme Oxygenase-1 by Oxidative Stress. The Journal of Biological Chemistry. 277: 9226-32.Whitby FG, et al. (2002) Crystal Structure of a Biliverdin IX Reductase Enzyme-Cofactor Complex. Journal of Molecular Biology. 319(5): 1199-210.

NCBI and Uniprot Product Information

• NCBI GI #: 1706870
• NCBI GeneID: 645
• UniProt Accession #: P30043; P32078; P53005; Q32LZ2; A6NKD8; B2R5C6
• Molecular Weight: 206
• NCBI Official Full Name: Flavin reductase (NADPH)
• NCBI Official Synonym Full Names: biliverdin reductase B
• NCBI Official Symbol: BLVRB
• NCBI Official Synonym Symbols: FLR; BVRB; SDR43U1; HEL-S-10
• NCBI Protein Information: flavin reductase (NADPH); flavin reductase (NADPH); FR; GHBP; BVR-B; NADPH-flavin reductase; NADPH-dependent diaphorase; green heme-binding protein; biliverdin-IX beta-reductase; epididymis secretory protein Li 10; biliverdin reductase B (flavin reductase
• UniProt Protein Name: Flavin reductase (NADPH)
• UniProt Gene Name: BLVRB
• UniProt Synonym Gene Names: FLR; FR; BVR-B; GHBP; FLR
• UniProt Entry Name: BLVRB_HUMAN

NCBI Description

The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC 1.3.1.24).[supplied by OMIM, Jul 2009]

Uniprot Description

BLVRB: Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase; EC 1.5.1.30; EC 1.3.1.24

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: flavin reductase activity; biliverdin reductase activity

Biological Process: heme catabolic process; porphyrin metabolic process