Rabbit BLK Polyclonal Antibody | anti-BLK antibody

BLK (Phospho-Tyr389) Antibody

Cat. Num. AAA9416577

• Gene Names: BLK; MODY11
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: BLK; Polyclonal Antibody; BLK (Phospho-Tyr389) Antibody; Blk kinase; B lymphocyte kinase; B lymphoid tyrosine kinase; MODY11; Protein kinase blk; Protein tyrosine kinase blk; B lymphoid kinase; p55-Blk; Tyrosine-protein kinase Blk; anti-BLK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: BLK (Phospho-Tyr389) Antibody detects endogenous levels of BLK only when phosphorylated at Tyr389
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 505

• Applicable Applications for anti-BLK antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: A synthesized peptide derived from human BLK (Phospho-Tyr389)
• Immunogen Type: Peptide
• Modification: Phospho-Tyr389
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis BLK (Phospho-Tyr389) using PI3-kina H2O2 treated COS7 whole cell lysates)

Product Categories/Family for anti-BLK antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 33469982
• NCBI GeneID: 640
• NCBI Accession #: NP_001706.2
• NCBI GenBank Nucleotide #: NM_001715.2
• UniProt Accession #: P51451; Q16291; Q96IN1
• Molecular Weight: 57,706 Da
• NCBI Official Full Name: tyrosine-protein kinase Blk isoform 1
• NCBI Official Synonym Full Names: BLK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: BLK
• NCBI Official Synonym Symbols: MODY11
• NCBI Protein Information: tyrosine-protein kinase Blk
• UniProt Protein Name: Tyrosine-protein kinase Blk
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BLK
• UniProt Entry Name: BLK_HUMAN

NCBI Description

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Uniprot Description

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Src family; TK group

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: B cell receptor signaling pathway; cell differentiation; cytoskeleton organization and biogenesis; innate immune response; negative regulation of apoptosis; positive regulation of insulin secretion; regulation of cell proliferation; regulation of phagocytosis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Maturity-onset Diabetes Of The Young, Type 11