Rabbit BLK Polyclonal Antibody | anti-BLK antibody

BLK Rabbit Polyclonal

Cat. Num. AAA767138

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: BLK; Polyclonal Antibody; BLK Rabbit Polyclonal; anti-BLK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 499

• Applicable Applications for anti-BLK antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: B lymphoid tyrosine kinase
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreas cancer using MBS767138 (BLK antibody) at dilution of 1:10)

Testing Data

()

Western Blot (WB)

(SH-SY5Y cells were subjected to SDS PAGE followed by western blot with MBS767138 (BLK antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 71059809
• NCBI GeneID: 12143
• UniProt Accession #: Q8K2M8; P16277
• Molecular Weight: 56,674 Da
• NCBI Official Full Name: Blk
• NCBI Official Synonym Full Names: B lymphoid kinase
• NCBI Official Symbol: Blk
• NCBI Protein Information: tyrosine-protein kinase Blk
• UniProt Protein Name: Tyrosine-protein kinase Blk
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Blk
• UniProt Entry Name: BLK_MOUSE

Uniprot Description

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptor FCGR2. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.