Rabbit anti-Human BLIMP1 Polyclonal Antibody | anti-PRDM1 antibody

BLIMP1, CT (PRDM1, PR Domain Zinc Finger Protein 1, BLIMP-1, Beta-interferon Gene Positive Regulatory Domain I-binding Factor, PR Domain-containing Protein 1, Positive Regulatory Domain I-binding Factor 1, PRDI-BF1, PRDI-binding factor 1)

Cat. Num. AAA6010086

• Gene Names: PRDM1; BLIMP1; PRDI-BF1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: BLIMP1; Polyclonal Antibody; CT (PRDM1; PR Domain Zinc Finger Protein 1; BLIMP-1; Beta-interferon Gene Positive Regulatory Domain I-binding Factor; PR Domain-containing Protein 1; Positive Regulatory Domain I-binding Factor 1; PRDI-BF1; PRDI-binding factor 1); Anti -BLIMP1; anti-PRDM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human PRDM1. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-PRDM1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.,; Dilution: Immunofluorescence: 20ug/ml; Western Blot: 0.5-1ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml
• Immunogen: Synthetic peptide corresponding to 13aa from near the C-terminal of human BLIMP1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Colon, Lymphoid Follicle (formalin-fixed, paraffin-embedded) stained with PRDM1 antibody at 3ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Western blot analysis of Blimp-1 in A549 cell lysate with Blimp-1 antibody at (A) 0.5 and (B) 1ug/ml.)

Related Product Information for anti-PRDM1 antibody

This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.

Product Categories/Family for anti-PRDM1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 3493158
• NCBI GeneID: 639
• UniProt Accession #: O75626; Q86WM7; B2REA6; E1P5E0
• Molecular Weight: 91,771 Da
• NCBI Official Full Name: beta-interferon gene positive-regulatory domain I binding factor
• NCBI Official Synonym Full Names: PR domain containing 1, with ZNF domain
• NCBI Official Symbol: PRDM1
• NCBI Official Synonym Symbols: BLIMP1; PRDI-BF1
• NCBI Protein Information: PR domain zinc finger protein 1; BLIMP-1; PRDI-binding factor-1; B-lymphocyte-induced maturation protein 1; beta-interferon gene positive-regulatory domain I binding factor
• UniProt Protein Name: PR domain zinc finger protein 1
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: PRDM1
• UniProt Synonym Gene Names: BLIMP1; PRDI-BF1
• UniProt Entry Name: PRDM1_HUMAN

NCBI Description

This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDM1: a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene and can inhibit virus-mediated IFN-¿ production. Recruits chromatin-modifying enzymes including histone deacetylases and methyltransferases. Drives the maturation of B-lymphocytes into Ig secreting cells. Interacts with PRMT5. Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1. In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation and in a subset of T-cells suggesting that it may play a wider role in homeostasis and differentiation. Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc, CIITA, Pax5, Spi-B, and Id3.Three isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Methyltransferase, protein lysine, predicted; Transcription regulation; DNA-binding

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; nucleus

Molecular Function: methyltransferase activity; protein binding; metal ion binding; histone deacetylase binding; transcription factor activity

Biological Process: methylation; negative regulation of lipopolysaccharide-mediated signaling pathway; eye photoreceptor cell development; cell fate commitment; transcription, DNA-dependent; positive regulation of B cell differentiation; negative regulation of B cell proliferation; negative regulation of transcription from RNA polymerase II promoter; post-embryonic development; germ cell development; maternal placenta development