Rabbit anti-Human BIRC3 Polyclonal Antibody | anti-BIRC3 antibody

BIRC3, NT (BIRC3, API2, IAP1, MIHC, RNF49, Baculoviral IAP repeat-containing protein 3, Apoptosis inhibitor 2, C-IAP2, IAP homolog C, Inhibitor of apoptosis protein 1, RING finger protein 49, TNFR2-TRAF-signaling complex protein 1) (APC)

Cat. Num. AAA6278451

• Gene Names: BIRC3; AIP1; API2; MIHC; CIAP2; HAIP1; HIAP1; MALT2; RNF49; c-IAP2
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BIRC3; Polyclonal Antibody; NT (BIRC3; API2; IAP1; MIHC; RNF49; Baculoviral IAP repeat-containing protein 3; Apoptosis inhibitor 2; C-IAP2; IAP homolog C; Inhibitor of apoptosis protein 1; RING finger protein 49; TNFR2-TRAF-signaling complex protein 1) (APC); TNFR2-TRAF-signaling complex protein 1; anti-BIRC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-BIRC3 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BIRC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-88 amino acids from the N-terminal region of human BIRC3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(BIRC3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the BIRC3 antibody detected the BIRC3 protein (arrow).)

Western Blot (WB)

(Western blot analysis of BIRC3 (arrow) using rabbit polyclonal BIRC3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BIRC3 gene.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of BIRC3 Antibody (N-term) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(BIRC3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BIRC3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-BIRC3 antibody

The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. The amino acid sequence predicts three baculovirus IAP repeat domains and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq].

Product Categories/Family for anti-BIRC3 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Zane, L., et al. Virology 407(2):341-351(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Kim, C.W., et al. Biochem. Biophys. Res. Commun. 400(1):46-52(2010). Petersen, S.L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11936-11941(2010). Friboulet, L., et al. BMC Cancer 10, 327 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4502139
• NCBI GeneID: 330
• NCBI Accession #: NP_001156.1; NP_892007.1
• NCBI GenBank Nucleotide #: NM_001165.4
• UniProt Accession #: Q13489; Q16628; Q9HC27; Q9UP46
• Molecular Weight: 68,372 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 3
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 3
• NCBI Official Symbol: BIRC3
• NCBI Official Synonym Symbols: AIP1; API2; MIHC; CIAP2; HAIP1; HIAP1; MALT2; RNF49; c-IAP2
• NCBI Protein Information: baculoviral IAP repeat-containing protein 3
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 3
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC3
• UniProt Synonym Gene Names: API2; IAP1; MIHC; RNF49; API2; IAP-1; hIAP-1; hIAP1
• UniProt Entry Name: BIRC3_HUMAN

NCBI Description

This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

cIAP2: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase- independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). Interacts with RIP1, RIP2, RIP3, RIP4 and USP19. Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes. USP19 regulates the stability of BIRC3/c-IAP2 by preventing its ubiquitination. Belongs to the IAP family.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ligase; Apoptosis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q22

Cellular Component: nucleoplasm; spindle microtubule; cytoplasm; nucleus; cytosol; lipid raft

Molecular Function: protein binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: regulation of toll-like receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; MyD88-independent toll-like receptor signaling pathway; protein ubiquitination; toll-like receptor 3 signaling pathway; regulation of innate immune response; regulation of apoptosis; cell surface receptor linked signal transduction; positive regulation of protein ubiquitination; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; negative regulation of phosphorylation; spermatogenesis; toll-like receptor 4 signaling pathway; negative regulation of apoptosis