Western Blot (WB)
(All lanes : Anti-beta II Tubulin at 1:8000 dilutionLane 1: mouse brain lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: PC-12 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit beta II Tubulin Polyclonal Antibody | anti-Tubb2a antibody
beta II Tubulin Antibody
Gene Names
Tubb2a; Tubb2; M(beta)2
Reactivity
Human, Mouse, Rat; Predicted Reactivity: Human, Mouse, Rat, Pig, Chicken, Bovine
Applications
Western Blot, ELISA
Synonyms
beta II Tubulin; Polyclonal Antibody; beta II Tubulin Antibody; Tubulin beta-2A chain; Tubb2a; Tubb2; anti-Tubb2a antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat; Predicted Reactivity: Human, Mouse, Rat, Pig, Chicken, Bovine
Clonality
Polyclonal
Isotype
Ig
Specificity
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-46 amino acids from human.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
445
Applicable Applications for anti-Tubb2a antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm, cytoskeleton.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-beta II Tubulin at 1:8000 dilutionLane 1: mouse brain lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: PC-12 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-beta II Tubulin at 1:8000 dilutionLane 1: mouse brain lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: PC-12 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-beta II Tubulin at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLane 3: NIH/3T3 whole cell lysateLane 4: PC-12 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-beta II Tubulin at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLane 3: NIH/3T3 whole cell lysateLane 4: PC-12 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Tubb2a antibody
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Product Categories/Family for anti-Tubb2a antibody
References
Carninci P.,et al.Science 309:1559-1563 (2005). Lubec G.,et al.Submitted (JAN-2009) to UniProtKB. Janke C.,et al.Science 308:1758-1762 (2005). Rogowski K.,et al.Cell 137:1076-1087 (2009). Yoshida K.,et al.Biochem. Biophys. Res. Commun. 389:506-511 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49907 Da
NCBI Official Full Name
tubulin beta-2A chain
NCBI Official Synonym Full Names
tubulin, beta 2A class IIA
NCBI Official Symbol
Tubb2a
NCBI Official Synonym Symbols
Tubb2; M(beta)2
NCBI Protein Information
tubulin beta-2A chain
UniProt Protein Name
Tubulin beta-2A chain
Protein Family
UniProt Gene Name
Tubb2a
UniProt Synonym Gene Names
Tubb2
UniProt Entry Name
TBB2A_MOUSE
Uniprot Description
TUBB2A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Cellular Component: cytoplasm; cytoskeleton; microtubule; nucleus
Molecular Function: GTP binding; GTPase activity; nucleotide binding; structural constituent of cytoskeleton
Biological Process: microtubule-based process
Research Articles on Tubb2a
Similar Products
Product Notes
The Tubb2a tubb2a (Catalog #AAA9217261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta II Tubulin Antibody reacts with Human, Mouse, Rat; Predicted Reactivity: Human, Mouse, Rat, Pig, Chicken, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's beta II Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the Tubb2a tubb2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta II Tubulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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