Western Blot (WB)
(BEND4 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the BEND4 antibody detected the BEND4 protein (arrow).)
Rabbit anti-Human BEND4 Polyclonal Antibody | anti-BEND4 antibody
BEND4, NT (BEND4, CCDC4, BEN domain-containing protein 4, Coiled-coil domain-containing protein 4) (Azide free) (HRP)
Gene Names
BEND4; CCDC4
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
BEND4; Polyclonal Antibody; NT (BEND4; CCDC4; BEN domain-containing protein 4; Coiled-coil domain-containing protein 4) (Azide free) (HRP); Coiled-coil domain-containing protein 4; anti-BEND4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-BEND4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
BEND4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human BEND4.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(BEND4 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the BEND4 antibody detected the BEND4 protein (arrow).)
Western Blot (WB)
(BEND4 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the BEND4 antibody detected the BEND4 protein (arrow).)
Flow Cytometry (FC/FACS)
(BEND4 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(BEND4 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-BEND4 antibody
References
Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,810 Da
NCBI Official Full Name
BEN domain-containing protein 4 isoform a
NCBI Official Synonym Full Names
BEN domain containing 4
NCBI Official Symbol
BEND4
NCBI Official Synonym Symbols
CCDC4
NCBI Protein Information
BEN domain-containing protein 4; coiled-coil domain containing 4; coiled-coil domain-containing protein 4
UniProt Protein Name
BEN domain-containing protein 4
Protein Family
UniProt Gene Name
BEND4
UniProt Synonym Gene Names
CCDC4
UniProt Entry Name
BEND4_HUMAN
Uniprot Description
CCDC4: 5 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 4p13
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Product Notes
The BEND4 bend4 (Catalog #AAA6278223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BEND4, NT (BEND4, CCDC4, BEN domain-containing protein 4, Coiled-coil domain-containing protein 4) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BEND4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BEND4 bend4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BEND4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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