Rabbit anti-Human, Mouse Beclin1 Polyclonal Antibody | anti-BECN1 antibody

Beclin1, BH3 Domain (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) (Biotin)

Cat. Num. AAA6278155

• Gene Names: BECN1; ATG6; VPS30; beclin1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Beclin1; Polyclonal Antibody; BH3 Domain (BECN1; GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197) (Biotin); BECN1; Protein GT197; anti-BECN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Beclin1-BH3. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-BECN1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 95-132 of human Beclin1-BH3, UniProt Accession #Q14457. Species sequence homology: bovine, chicken, porcine, rat, xenopus
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of in mouse stomach tissue lysates (35ug/lane) usingMBS644337. BECN1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue using MBS644337 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of Hela cells (right histogram) using MBS644337 compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-BECN1 antibody

Beclin1-BH3 plays a central role in autophagy (By similarity). It may play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Product Categories/Family for anti-BECN1 antibody

Antibodies; Autophagy

References

Won, K.Y., et.al., Hum. Pathol. 41 (1), 107-112 (2010). Oberstein, A., et.al., J. Biol. Chem. 282 (17), 13123-13132 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4680381
• NCBI GeneID: 8678
• UniProt Accession #: Q14457; O75595; Q9UNA8
• Molecular Weight: 51,896 Da
• NCBI Official Full Name: beclin 1
• NCBI Official Synonym Full Names: beclin 1, autophagy related
• NCBI Official Symbol: BECN1
• NCBI Official Synonym Symbols: ATG6; VPS30; beclin1
• NCBI Protein Information: beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
• UniProt Protein Name: Beclin-1
• Protein Family: Beclin
• UniProt Gene Name: BECN1
• UniProt Synonym Gene Names: GT197
• UniProt Entry Name: BECN1_HUMAN

NCBI Description

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

Uniprot Description

beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Protein type: Adaptor/scaffold; Autophagy; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; dendrite; phagocytic vesicle; trans-Golgi network; nucleus; endosome

Molecular Function: protein binding; phosphoinositide 3-kinase binding

Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; lysosome organization and biogenesis; regulation of catalytic activity; beta-amyloid metabolic process; mitochondrion degradation; response to hypoxia; cellular defense response; neuron development; defense response to virus; positive regulation of macroautophagy; positive regulation of autophagy; negative regulation of apoptosis; autophagic vacuole formation