Rabbit anti-Human Beclin-1 Polyclonal Antibody | anti-BECN1 antibody

Beclin-1, NT (T72) (Coiled-coil Myosin-like BCL2-interacting Protein, Protein GT197, BECN1, GT197) (MaxLight 750)

Cat. Num. AAA6278195

• Gene Names: BECN1; ATG6; VPS30; beclin1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Beclin-1; Polyclonal Antibody; NT (T72) (Coiled-coil Myosin-like BCL2-interacting Protein; Protein GT197; BECN1; GT197) (MaxLight 750); GT197; Coiled-coil myosin-like BCL2-interacting protein; anti-BECN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Beclin-1
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-BECN1 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 52-78 in the N-terminal region of human Beclin-1, Swiss/UniProt Accession: Q14457.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6002033. This demonstrates that MBS6002033 detected the BECN1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in Hela whole cell lysate (20ug) using MBS6002033 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 52kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-BECN1 antibody

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM].

Product Categories/Family for anti-BECN1 antibody

Antibodies; Autophagy

References

1. Koukourakis, M.I., et al. Br. J. Cancer 103(8):1209-1214(2010). 2. Jaeger, P.A., et al. Arch. Neurol. 67(10):1181-1184(2010). 3. Metzger, S., et al. Hum. Genet. 128(4):453-459(2010). 4. Oberstein, A., et al. J. Biol. Chem. 282(17):13123-13132(2007). 5. Furuya, N., et al. Autophagy 1(1):46-52(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4502395
• NCBI GeneID: 8678
• NCBI Accession #: NP_003757.1
• NCBI GenBank Nucleotide #: NM_003766.3
• UniProt Accession #: Q14457; O75595; Q9UNA8
• Molecular Weight: 52kDa
• NCBI Official Full Name: beclin-1
• NCBI Official Synonym Full Names: beclin 1, autophagy related
• NCBI Official Symbol: BECN1
• NCBI Official Synonym Symbols: ATG6; VPS30; beclin1
• NCBI Protein Information: beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
• UniProt Protein Name: Beclin-1
• Protein Family: Beclin
• UniProt Gene Name: BECN1
• UniProt Synonym Gene Names: GT197
• UniProt Entry Name: BECN1_HUMAN

Uniprot Description

beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Protein type: Adaptor/scaffold; Autophagy; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; dendrite; phagocytic vesicle; trans-Golgi network; nucleus; endosome

Molecular Function: protein binding; phosphoinositide 3-kinase binding

Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; lysosome organization and biogenesis; regulation of catalytic activity; beta-amyloid metabolic process; mitochondrion degradation; response to hypoxia; cellular defense response; neuron development; defense response to virus; positive regulation of macroautophagy; positive regulation of autophagy; negative regulation of apoptosis; autophagic vacuole formation