Rabbit Beclin 1 Polyclonal Antibody | anti-BECN1 antibody

Beclin 1 Antibody

Cat. Num. AAA9211205

• Gene Names: BECN1; ATG6; VPS30; beclin1
• Reactivity: Human, mouse (Predicted Reactivity: Bovine, Chicken)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Beclin 1; Polyclonal Antibody; Beclin 1 Antibody; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197; anti-BECN1 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine, Chicken)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from human Beclin 1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 181-210
• Sequence Length: 450

• Applicable Applications for anti-BECN1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-hBECN1-Q196 Pab in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-Q196(arrow) was detected using the purified Pab.)

Western Blot (WB)

(The anti-BECN1 Pab is used in Western blot to detect BECN1 in mouse liver tissue lysate. BECN1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-BECN1 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.

Product Categories/Family for anti-BECN1 antibody

Cancer; Metabolism; Microbiology; Autophagy

References

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) Liang,X.H., et al. J. Virol. 72 (11), 8586-8596 (1998) Aita V.M., et al. Genomics 59:59-65(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4502395
• NCBI GeneID: 8678
• NCBI Accession #: NP_003757.1
• NCBI GenBank Nucleotide #: NM_003766.3
• UniProt Accession #: Q14457; O75595; Q9UNA8; B2R6N7
• Molecular Weight: 51896
• NCBI Official Full Name: beclin-1
• NCBI Official Synonym Full Names: beclin 1, autophagy related
• NCBI Official Symbol: BECN1
• NCBI Official Synonym Symbols: ATG6; VPS30; beclin1
• NCBI Protein Information: beclin-1
• UniProt Protein Name: Beclin-1
• Protein Family: Beclin
• UniProt Gene Name: BECN1
• UniProt Synonym Gene Names: GT197
• UniProt Entry Name: BECN1_HUMAN

NCBI Description

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

Uniprot Description

beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Protein type: Autophagy; Membrane protein, peripheral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; dendrite; trans-Golgi network; phagocytic vesicle; nucleus; endosome

Molecular Function: protein binding; phosphoinositide 3-kinase binding

Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; regulation of catalytic activity; lysosome organization and biogenesis; beta-amyloid metabolic process; mitochondrion degradation; response to hypoxia; cellular defense response; neuron development; positive regulation of macroautophagy; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis; autophagic vacuole formation