Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat BDH2/DHRS6 Polyclonal Antibody | anti-BDH2 antibody
Goat anti-BDH2/DHRS6 (aa 60 to 71) Antibody
Gene Names
BDH2; DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BDH2/DHRS6; Polyclonal Antibody; Goat anti-BDH2/DHRS6 (aa 60 to 71) Antibody; Goat Anti-BDH2 / DHRS6 (aa 92 to 103) Antibody; BDH2; DHRS6; 3-hydroxybutyrate dehydrogenase; type 2; EFA6R; FLJ13261; PRO20933; UCPA-OR; UNQ6308; dehydrogenase/reductase (SDR family) member 6; oxidoreductase UCPA; BDH2 antibody; DHRS6 antibody; type 2 antibody; EFA6R antibody; FLJ13261 antibody; PRO20933 antibody; UCPA-OR antibody; UNQ6308 antibody; dehydrogenase/reductase (SDR family) member 6 antibody; oxidoreductase UCPA antibody; BDH2 / DHRS6 (aa 60 to 71); anti-BDH2 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
TKKKQIDQFANE
Sequence Length
245
Applicable Applications for anti-BDH2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 26kDa band observed in Human Brain (Cerebellum), Kidney and Liver lysates (calculated MW of 26.7kDa according to NP_064524.3). In transfected HEK293 transiently expressing BDH2 a band of approx.29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 26kDa band observed in Human Brain (Cerebellum), Kidney and Liver lysates (calculated MW of 26.7kDa according to NP_064524.3). In transfected HEK293 transiently expressing BDH2 a band of approx.29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-TKKKQIDQFANE, from the internal region of the protein sequence according to NP_064524.3.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing BDH2 (RC210586) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing BDH2 (RC210586) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,626 Da
NCBI Official Full Name
3-hydroxybutyrate dehydrogenase type 2
NCBI Official Synonym Full Names
3-hydroxybutyrate dehydrogenase, type 2
NCBI Official Symbol
BDH2
NCBI Official Synonym Symbols
DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933
NCBI Protein Information
3-hydroxybutyrate dehydrogenase type 2
UniProt Protein Name
3-hydroxybutyrate dehydrogenase type 2
Protein Family
UniProt Gene Name
BDH2
UniProt Synonym Gene Names
DHRS6; SDR15C1
UniProt Entry Name
BDH2_HUMAN
Uniprot Description
DHRS6: Dehydrogenase that mediates the formation of 2,5- dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.1.1.30; Oxidoreductase; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - butanoate
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: cytoplasm; cytosol; mitochondrion
Molecular Function: 3-hydroxybutyrate dehydrogenase activity; NAD binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
Biological Process: epithelial cell differentiation; fatty acid beta-oxidation; heme metabolic process; iron ion homeostasis; ketone body biosynthetic process; siderophore biosynthetic process
Research Articles on BDH2
Similar Products
Product Notes
The BDH2 bdh2 (Catalog #AAA421578) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BDH2/DHRS6 (aa 60 to 71) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BDH2/DHRS6 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 26kDa band observed in Human Brain (Cerebellum), Kidney and Liver lysates (calculated MW of 26.7kDa according to NP_064524.3). In transfected HEK293 transiently expressing BDH2 a band of approx.29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the BDH2 bdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TKKKQIDQFA NE. It is sometimes possible for the material contained within the vial of "BDH2/DHRS6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
