Rabbit anti-Human BCL6 Polyclonal Antibody | anti-BCL6 antibody

BCL6, CT (BCL6, BCL5, LAZ3, ZBTB27, ZNF51, B Cell lymphoma 6 protein, B Cell lymphoma 5 protein, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51) (Azide free) (HRP)

Cat. Num. AAA6278003

• Gene Names: BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BCL6; Polyclonal Antibody; CT (BCL6; BCL5; LAZ3; ZBTB27; ZNF51; B Cell lymphoma 6 protein; B Cell lymphoma 5 protein; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51) (Azide free) (HRP); B-cell lymphoma 6 protein; B-cell lymphoma 5 protein; Zinc finger protein 51; anti-BCL6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BCL6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BCL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 675-704 amino acids from the C-terminal region of human BCL6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BCL6 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BCL6 antibody detected the BCL6 protein (arrow).)

Related Product Information for anti-BCL6 antibody

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

Product Categories/Family for anti-BCL6 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Chang, C.C., et al. J. Immunol. 185(10):5714-5722(2010). Yang, J., et al. J. Biol. Chem. 285(39):29760-29769(2010). Lai, A.Y., et al. J. Exp. Med. 207(9):1939-1950(2010). Ramachandrareddy, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11930-11935(2010). Gomyo, H., et al. Hematology 15(3):157-161(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 197927147
• NCBI GeneID: 604
• NCBI Accession #: NP_001128210.1; NP_001124317.1; NP_001697.2
• NCBI GenBank Nucleotide #: NM_001134738.1
• UniProt Accession #: P41182; A7E241; B8PSA7; D3DNV5
• Molecular Weight: 78 kDa
• NCBI Official Full Name: B-cell lymphoma 6 protein isoform 2
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 6
• NCBI Official Symbol: BCL6
• NCBI Official Synonym Symbols: BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
• NCBI Protein Information: B-cell lymphoma 6 protein; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; cys-his2 zinc finger transcription factor; lymphoma-associated zinc finger gene on chromosome 3; protein LAZ-3; zinc finger and BTB domain-containing
• UniProt Protein Name: B-cell lymphoma 6 protein
• Protein Family: B-cell lymphoma 6 protein
• UniProt Gene Name: BCL6
• UniProt Synonym Gene Names: BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5
• UniProt Entry Name: BCL6_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

Bcl-6: a zinc finger transcription factor and contains an N-terminal BTB/POZ domain. A sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. Mediates transcriptional repression and interacts with components of histone deacetylase co-repressor complexes including N-CoR and SMRT. The gene is frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL.

Protein type: C2H2-type zinc finger protein; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: replication fork; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; chromatin DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: erythrocyte development; positive regulation of apoptosis; regulation of memory T cell differentiation; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; Rho protein signal transduction; negative regulation of cell proliferation; negative regulation of isotype switching to IgE isotypes; positive regulation of histone deacetylation; positive regulation of B cell proliferation; germinal center formation; negative regulation of mast cell cytokine production; inflammatory response; negative regulation of T-helper 2 cell differentiation; regulation of immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of cell motility; negative regulation of cell-matrix adhesion; regulation of germinal center formation; negative regulation of Rho protein signal transduction; protein import into nucleus, translocation; B cell differentiation; regulation of inflammatory response; negative regulation of Notch signaling pathway; spermatogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation; actin cytoskeleton organization and biogenesis; response to DNA damage stimulus; negative regulation of B cell apoptosis