Rabbit anti-Human, Mouse Bcl-w Polyclonal Antibody | anti-Bcl-w antibody

Bcl-w, BH3 Domain (Bcl2-like Protein 2, BCL2L2, Apoptosis Regulator Bcl-W, BCLW, KIAA0271) (PE)

Cat. Num. AAA6465085

• Gene Names: BCL2L2; BCLW; BCL-W; PPP1R51; BCL2-L-2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Bcl-w; Polyclonal Antibody; BH3 Domain (Bcl2-like Protein 2; BCL2L2; Apoptosis Regulator Bcl-W; BCLW; KIAA0271) (PE); anti-Bcl-w antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bcl-w. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Bcl-w antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding within amino acid residues 1-100 of human Bcl-W (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Bcl-w BH3 domain Pab in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of Bcl-w3 (arrow) using rabbit polyclonal Bcl-w BH3 Domain Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Bcl-w BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(BCLW Antibody (BH3) (RB00972) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the BCLW antibody detected the BCLW protein (arrow).)

Product Categories/Family for anti-Bcl-w antibody

Antibodies; Apoptosis

References

1. Denisov, A.Y., et al., J. Biol. Chem. 278(23):21124-21128 (2003). 2. Hinds, M.G., et al., EMBO J. 22(7):1497-1507 (2003). 3. Middleton, G., et al., Development 128(3):447-457 (2001). 4. Ross, A.J., et al., Nat. Genet. 18(3):251-256 (1998). 5. Gibson, L., et al., Oncogene 13(4):665-675 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4757842
• NCBI GeneID: 599
• NCBI Accession #: NP_004041
• NCBI GenBank Nucleotide #: NM_004050.4
• UniProt Accession #: Q92843; Q2M3U0; Q5U0H4; A8K0F4
• Molecular Weight: 20.9 kDa (192aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: bcl-2-like protein 2
• NCBI Official Synonym Full Names: BCL2 like 2
• NCBI Official Symbol: BCL2L2
• NCBI Official Synonym Symbols: BCLW; BCL-W; PPP1R51; BCL2-L-2
• NCBI Protein Information: bcl-2-like protein 2
• UniProt Protein Name: Bcl-2-like protein 2
• UniProt Gene Name: BCL2L2
• UniProt Synonym Gene Names: BCLW; KIAA0271; Bcl2-L-2
• UniProt Entry Name: B2CL2_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BCL2L2: Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. Expressed in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: mitochondrial outer membrane; cytosol

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; Sertoli cell proliferation; spermatogenesis; negative regulation of apoptosis