Rabbit anti-Human BCHE Polyclonal Antibody | anti-BCHE antibody

BCHE, ID (Pseudocholinesterase, Butyrylcholine Esterase, Choline Esterase II, Acylcholine Acylhydrolase, Cholinesterase, CHE1) (PE)

Cat. Num. AAA6466105

• Gene Names: BCHE; E1; CHE1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: BCHE; Polyclonal Antibody; ID (Pseudocholinesterase; Butyrylcholine Esterase; Choline Esterase II; Acylcholine Acylhydrolase; Cholinesterase; CHE1) (PE); anti-BCHE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BCHE.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-BCHE antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human BCHE (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-BCHE Antibody (Center) in A2058 cell line lysates (35ug/lane). BCHE (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-BCHE antibody

Antibodies; Enzymes

References

1. Primo-Parmo S.L., Bartels C.F.Am. J. Hum. Genet. 58:52-64(1996). 2. Primo-Parmo S.L., Lightstone H.Pharmacogenetics 7:27-34(1997). 3. Yen T., Nightingale B.N.Clin. Chem. 49:1297-1308(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4557351
• NCBI GeneID: 590
• NCBI Accession #: NP_000046
• NCBI GenBank Nucleotide #: NM_000055
• UniProt Accession #: P06276; A8K7P8
• Molecular Weight: 68,418 Da
• NCBI Official Full Name: cholinesterase
• NCBI Official Synonym Full Names: butyrylcholinesterase
• NCBI Official Symbol: BCHE
• NCBI Official Synonym Symbols: E1; CHE1
• NCBI Protein Information: cholinesterase; cholinesterase 1; choline esterase II; pseudocholinesterase; butyrylcholine esterase; acylcholine acylhydrolase
• UniProt Protein Name: Cholinesterase
• Protein Family: Cholinesterase
• UniProt Gene Name: BCHE
• UniProt Synonym Gene Names: CHE1
• UniProt Entry Name: CHLE_HUMAN

Uniprot Description

BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family.

Protein type: Secreted, signal peptide; Hydrolase; Nuclear envelope; Secreted; EC 3.1.1.8

Chromosomal Location of Human Ortholog: 3q26.1-q26.2

Cellular Component: membrane; nuclear envelope lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function: identical protein binding; choline binding; enzyme binding; cholinesterase activity; beta-amyloid binding; acetylcholinesterase activity; catalytic activity

Biological Process: response to drug; response to alkaloid; negative regulation of cell proliferation; choline metabolic process; cocaine metabolic process; cellular protein metabolic process; response to folic acid; response to glucocorticoid stimulus; neuroblast differentiation; learning; negative regulation of synaptic transmission