Rabbit BCAR1 Polyclonal Antibody | anti-BCAR1 antibody

BCAR1 Conjugated Antibody

Cat. Num. AAA9447591

• Gene Names: BAG6; G3; BAT3; BAG-6; D6S52E
• Reactivity: Human, Mouse, Rat
• Applications: Most Applications
• Synonyms: BCAR1; Polyclonal Antibody; BCAR1 Conjugated Antibody; CAS; CAS1; CASS1; CRKAS; P130Cas; anti-BCAR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total BCAR1 protein.
• Form/Format: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide

• Applicable Applications for anti-BCAR1 antibody: Most Applications
• Application Notes: Suggested Dilution:; AF350 conjugated: most applications: 1:50-1:250; AF405 conjugated: most applications: 1:50-1:250; AF488 conjugated: most applications: 1:50-1:250; AF555 conjugated: most applications: 1:50-1:250; AF594 conjugated: most applications: 1:50-1:250; AF647 conjugated: most applications: 1:50-1:250; AF680 conjugated: most applications: 1:50-1:250; AF750 conjugated: most applications: 1:50-1:250; Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
• Immunogen: Synthetic peptide corresponding to residues near the N terminal of human breast cancer anti-estrogen resistance 1
• Conjugation: Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation Emission: AF350: 346nm/442nm
• Preparation and Storage: Store at 4 degree C for 6 months.

Related Product Information for anti-BCAR1 antibody

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.

NCBI and Uniprot Product Information

• NCBI GI #: 149158692
• NCBI GeneID: 7917
• NCBI Accession #: NP_004630
• NCBI GenBank Nucleotide #: NM_004639
• UniProt Accession #: P56945; P46379
• Molecular Weight: 125kDa
• NCBI Official Full Name: large proline-rich protein BAG6 isoform a
• NCBI Official Synonym Full Names: BCL2 associated athanogene 6
• NCBI Official Symbol: BAG6
• NCBI Official Synonym Symbols: G3; BAT3; BAG-6; D6S52E
• NCBI Protein Information: large proline-rich protein BAG6
• UniProt Protein Name: Breast cancer anti-estrogen resistance protein 1
• Protein Family: Breast cancer anti-estrogen resistance protein
• UniProt Gene Name: BCAR1
• UniProt Synonym Gene Names: CAS; CASS1; CRKAS
• UniProt Entry Name: BCAR1_HUMAN

NCBI Description

This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. Ref.10 Ref.11

Subunit structure: Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1 and SH2D3C. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1. Part of a collagen-stimulated complex involved in cell migration made of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with TNK2 via SH3 domains. Interacts with PTK2B/PYK2. Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.16

Subcellular location: Cell junction › focal adhesion. Cytoplasm. Note: Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation

By similarity. Ref.10

Tissue specificity: Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

Domain: Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9

By similarity.A serine-rich region promotes activation of the serum response element (SRE).The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.

Post-translational modification: PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Ref.10 Ref.13 Ref.15 Ref.20Dephosphorylated by PTPN14 at Tyr-128. Ref.10 Ref.13 Ref.15 Ref.20

Sequence similarities: Belongs to the CAS family.Contains 1 SH3 domain.