Western Blot (WB)
((1ug/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat BCAR1 Polyclonal Antibody | anti-BCAR1 antibody
Goat anti-BCAR1 Antibody
Gene Names
BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BCAR1; Polyclonal Antibody; Goat anti-BCAR1 Antibody; breast cancer anti-estrogen resistance 1; CAS; CAS1; CASS1; CRKAS; P130Cas; Cas scaffolding protein family member 1; Crk-associated substrate p130Cas; breast cancer anti-estrogen resistance protein 1; BCAR1 antibody; breast cancer anti-estrogen resistance 1 antibody; CAS antibody; CAS1 antibody; CASS1 antibody; CRKAS antibody; P130Cas antibody; Cas scaffolding protein family member 1 antibody; Crk-associated substrate p130Cas antibody; breast cancer anti-estrogen resistance protein 1 antibody; anti-BCAR1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KQTSTFSKQTPHH
Sequence Length
916
Applicable Applications for anti-BCAR1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 100kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9kDa according to NP_001164185.1). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 100kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9kDa according to NP_001164185.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-KQTSTFSKQTPHH, from the internal region of the protein sequence according to NP_001164185.1; NP_001164186.1; NP_001164187.1; NP_001164188.1; NP_001164189.1; NP_055382.2; NP_001164190.1; NP_001164191.1; NP_001164192.1.
Epitope
Internal region
Note
This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of MCF7 (A) and Human Breast cancer (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,291 Da
NCBI Official Full Name
breast cancer anti-estrogen resistance protein 1 isoform 1
NCBI Official Synonym Full Names
BCAR1, Cas family scaffolding protein
NCBI Official Symbol
BCAR1
NCBI Official Synonym Symbols
CAS; CAS1; CASS1; CRKAS; P130Cas
NCBI Protein Information
breast cancer anti-estrogen resistance protein 1
UniProt Protein Name
Breast cancer anti-estrogen resistance protein 1
Protein Family
UniProt Gene Name
BCAR1
UniProt Synonym Gene Names
CAS; CASS1; CRKAS
UniProt Entry Name
BCAR1_HUMAN
NCBI Description
BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009]
Uniprot Description
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
Research Articles on BCAR1
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Product Notes
The BCAR1 bcar1 (Catalog #AAA423291) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BCAR1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's BCAR1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10ug/ml. Western Blot: Approx 100kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9kDa according to NP_001164185.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the BCAR1 bcar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KQTSTFSKQT PHH. It is sometimes possible for the material contained within the vial of "BCAR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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