Rabbit anti-Human BARD1 Polyclonal Antibody | anti-BARD1 antibody

Anti-BARD1 Antibody

Cat. Num. AAA8216115

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: BARD1; Polyclonal Antibody; Anti-BARD1 Antibody; BRCA1-associated RING domain protein 1; BARD-1; anti-BARD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of BARD1 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 127

• Applicable Applications for anti-BARD1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB (1/500 - 1/1000), IHC (1/100 - 1/200)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BARD1. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of BARD1 expression in MCF7 (A), HeLa (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of BARD1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-BARD1 antibody

Rabbit polyclonal antibody to BARD1

NCBI and Uniprot Product Information

• NCBI GI #: 157266328
• NCBI GeneID: 580
• NCBI Accession #: NP_000456.2
• NCBI GenBank Nucleotide #: NM_000465.3
• UniProt Accession #: Q99728; O43574; Q53SS5; F6MDH7; F6MDH8; F6MDH9
• Molecular Weight: 14,362 Da
• NCBI Official Full Name: BRCA1-associated RING domain protein 1 isoform 1
• NCBI Official Synonym Full Names: BRCA1 associated RING domain 1
• NCBI Official Symbol: BARD1
• NCBI Protein Information: BRCA1-associated RING domain protein 1
• UniProt Protein Name: BRCA1-associated RING domain protein 1
• Protein Family: BRCA1-associated RING domain protein
• UniProt Gene Name: BARD1
• UniProt Synonym Gene Names: BARD-1
• UniProt Entry Name: BARD1_HUMAN

NCBI Description

This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

BARD1: the BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage. Colocalizes with BRCA1 into discrete subnuclear foci during S phase. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex. Defects in BARD1 gene are found in primary breast, ovarian and uterine cancers.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; BRCA1-BARD1 complex; nucleolus; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; RNA binding; protein heterodimerization activity; ubiquitin-protein ligase activity; kinase binding; ligase activity

Biological Process: negative regulation of mRNA 3'-end processing; negative regulation of protein export from nucleus; regulation of phosphorylation; positive regulation of apoptosis; positive regulation of protein catabolic process; tissue homeostasis; protein ubiquitination; DNA repair; cell cycle arrest; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Breast Cancer