Rabbit Band 3 Polyclonal Antibody | anti-Band 3 antibody

Anti-Band 3 Antibody

Cat. Num. AAA177834

• Gene Names: SLC4A1; DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A
• Reactivity: Human, Mouse , Rat.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Band 3; Polyclonal Antibody; Anti-Band 3 Antibody; Band 3 anion transport protein; AE 1; AE1; Anion exchange protein 1; Anion exchanger 1; B3AT_HUMAN; BND3; CD233; DI; Diego blood group; EMPB3; EPB3; Erythrocyte membrane protein band 3; Erythroid anion exchange protein; FR; Froese blood group; RTA1A; SLC4A1; Solute carrier family 4 anion exchanger member 1; Solute carrier family 4 member 1; SW; Swann blood group; Waldner blood group; WD; WD1; WR; Wright blood group; solute carrier family 4 (anion exchanger); member 1 (Diego blood group); anti-Band 3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse , Rat.
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. ; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
• Sequence Length: 911

• Applicable Applications for anti-Band 3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Immunofluorescence:; Concentration: 2ug/ml; Tested Species: Hu, Rat; ; Western Blot:; Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Rat; ; Immunohistochemistry (Paraffin-embedded Section); Concentration: 0.5-1ug/ml; Tested Species: Hu, Ms, Rat; Antigen Retrieval: By Heat; ; Immunohistochemistry (Frozen Section); Concentration: 0.5-1ug/ml; Tested Species: Hu; ; ; Tested Species: In-house tested species with positive results; ; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.; ; Other applications have not been tested.; ; Optimal dilutions should be determined by end user.
• Immunogen: E Coli-derived human Band 3 (Position: E28-N365). Human Band 3 shares 75.7% and 74.5% amino acid (aa) sequence identity with mouse and rat Band 3, respectively.
• Gene Name: Solute carrier family 4 (annion exchanger), member 1 (Diego blood group)
• Recommended Protein Name: Band 3 anion transport protein.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Band 3 Picoband antibody, Western blottingAll lanes: Anti Band 3 at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: Rat Spleen Tissue Lysate at 50ugLane 5: Human Placenta Tissue Lysate at 50ugPredicted bind size: 102KDObserved bind size: 102KD)

Testing Data

(Anti- Band 3 Picoband antibody, PB9437,IHC(P)IHC(P): Rat Kidney Tissue)

Immunohistochemistry (IHC)

(Anti- Band 3 Picoband antibody,IHC(P)IHC(P): Human Placenta Tissue)

Immunohistochemistry (IHC)

(Anti- Band 3 Picoband antibody,IHC(P)IHC(P): Mouse Kidney Tissue)

Immunohistochemistry (IHC)

(Anti- Band 3 Picoband antibody, PB9437,IHC(F)IHC(F): Human Placenta Tissue)

Immunofluorescence (IF)

(Figure 6. IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

Immunofluorescence (IF)

(Figure 7. IF analysis of Band 3 using anti-Band 3 antibody Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-Band 3 Antibody overnight at 4 degree C. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

Related Product Information for anti-Band 3 antibody

Description: Rabbit IgG polyclonal antibody for Band 3 anion transport protein(SLC4A1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

Background: Band 3 is also known as SLC4A1. The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system.

References

1. Jarolim P, Palek J, Amato D, Hassan K, Sapak P, Nurse GT et al. (1991). "Deletion in erythrocyte band 3 gene in malaria-resistant Southeast Asian ovalocytosis".Proc. Natl. Acad. Sci. U.S.A. 88 (24): 11022-6. 2. Schlüter K, Drenckhahn D (August 1986)."Co-clustering of denatured hemoglobin with band 3: its role in binding of autoantibodies against band 3 to abnormal and aged erythrocytes". Proc. Natl. Acad. Sci. U.S.A. 83 (16): 6137-41.

NCBI and Uniprot Product Information

• NCBI GI #: 4507021
• NCBI GeneID: 6521
• NCBI Accession #: NP_000333.1
• NCBI GenBank Nucleotide #: NM_000342.3
• UniProt Accession #: P02730; P78487; Q1ZZ45; Q4KKW9; Q4VB84; Q9UCY7; Q9UDJ1; G4V2I6
• NCBI Official Full Name: band 3 anion transport protein
• NCBI Official Synonym Full Names: solute carrier family 4 member 1 (Diego blood group)
• NCBI Official Symbol: SLC4A1
• NCBI Official Synonym Symbols: DI; FR; SW; WD; WR; AE1; CHC; SAO; WD1; BND3; EPB3; SPH4; CD233; EMPB3; RTA1A
• NCBI Protein Information: band 3 anion transport protein
• UniProt Protein Name: Band 3 anion transport protein
• Protein Family: Band 3 anion exchange protein
• UniProt Gene Name: SLC4A1
• UniProt Synonym Gene Names: AE1; DI; EPB3; AE 1; Anion exchanger 1
• UniProt Entry Name: B3AT_HUMAN

NCBI Description

The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.