Rabbit BAG3 Polyclonal Antibody | anti-BAG3 antibody

BAG3 (Phospho-Tyr457) Antibody

Cat. Num. AAA9416663

• Gene Names: BAG3; BIS; MFM6; BAG-3; CAIR-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: BAG3; Polyclonal Antibody; BAG3 (Phospho-Tyr457) Antibody; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; BCL2-binding athanogene 3; BIS; BAG-3; CAIR-1; Docking protein cair1; BCL2-associated athanogene 3; Docking protein CAIR-1; anti-BAG3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: BAG3 (Phospho-Tyr457) Antibody detects endogenous levels of BAG3 only when phosphorylated at Tyr457
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 575

• Applicable Applications for anti-BAG3 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: A synthesized peptide derived from human BAG3 (Phospho-Tyr457)
• Immunogen Type: Peptide
• Modification: Phospho-Tyr457
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis BAG3 (Phospho-Tyr457) using HeLa whole cell lysates)

Product Categories/Family for anti-BAG3 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 14043024
• NCBI GeneID: 9531
• NCBI Accession #: NP_004272.2
• NCBI GenBank Nucleotide #: NM_004281.3
• UniProt Accession #: O95817; Q3B763; Q9NT20; Q9P120; A8K5L8
• Molecular Weight: 61,595 Da
• NCBI Official Full Name: BAG family molecular chaperone regulator 3
• NCBI Official Synonym Full Names: BCL2 associated athanogene 3
• NCBI Official Symbol: BAG3
• NCBI Official Synonym Symbols: BIS; MFM6; BAG-3; CAIR-1
• NCBI Protein Information: BAG family molecular chaperone regulator 3
• UniProt Protein Name: BAG family molecular chaperone regulator 3
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG3
• UniProt Synonym Gene Names: BIS; BAG-3
• UniProt Entry Name: BAG3_HUMAN

NCBI Description

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Uniprot Description

BAG3: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. Defects in BAG3 are the cause of myopathy myofibrillar type 6 (MFM6). A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. Defects in BAG3 are the cause of cardiomyopathy dilated type 1HH (CMD1HH). CMD1HH is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 10q25.2-q26.2

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; plasma membrane

Molecular Function: adenyl-nucleotide exchange factor activity; protein binding

Biological Process: induction of apoptosis via death domain receptors

Disease: Cardiomyopathy, Dilated, 1hh; Myopathy, Myofibrillar, 6