Rabbit anti-Human BAG1 Polyclonal Antibody | anti-BAG1 antibody

BAG1, CT (BAG Family Molecular Chaperone Regulator 1, BAG-1, Bcl-2-associated Athanogene 1, HAP)

Cat. Num. AAA6005673

• Gene Names: BAG1; HAP; BAG-1; RAP46
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: BAG1; Polyclonal Antibody; CT (BAG Family Molecular Chaperone Regulator 1; BAG-1; Bcl-2-associated Athanogene 1; HAP); Anti -BAG1; anti-BAG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human BAG-1. Species sequence homology: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-BAG1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 14aa from human BAG-1 at C-terminal (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with BAG1 antibody at 5ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-BAG1 antibody

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.

Product Categories/Family for anti-BAG1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 288915525
• NCBI GeneID: 573
• NCBI Accession #: NP_004314.5
• NCBI GenBank Nucleotide #: NM_004323.5
• UniProt Accession #: Q99933; O75315; Q14414; Q53H32; Q5VZE8; Q5VZE9; Q5VZF0; Q96TG2; Q9Y2V4
• Molecular Weight: 38,779 Da
• NCBI Official Full Name: BAG family molecular chaperone regulator 1 isoform BAG-1L
• NCBI Official Synonym Full Names: BCL2-associated athanogene
• NCBI Official Symbol: BAG1
• NCBI Official Synonym Symbols: HAP; BAG-1; RAP46
• NCBI Protein Information: BAG family molecular chaperone regulator 1; Bcl-2-binding protein; receptor-associated protein, 46-KD; Bcl-2 associating athanogene-1 protein; glucocortoid receptor-associated protein RAP46
• UniProt Protein Name: BAG family molecular chaperone regulator 1
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG1
• UniProt Synonym Gene Names: HAP; BAG-1
• UniProt Entry Name: BAG1_HUMAN

NCBI Description

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]

Uniprot Description

BAG1: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2. Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium. Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9p12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: cell surface receptor linked signal transduction; apoptosis; negative regulation of apoptosis