Rabbit BAFF Polyclonal Antibody | anti-TNFSF13B antibody

BAFF Antibody

Cat. Num. AAA150169

• Gene Names: TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: BAFF Antibody is Ion exchange chromatography purified.
• Synonyms: BAFF; Polyclonal Antibody; BAFF Antibody; DTL; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20; UNQ401/PRO738; Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; tumor necrosis factor (ligand) superfamily; member 13b; anti-TNFSF13B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: BAFF Antibody is Ion exchange chromatography purified.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 285

• Applicable Applications for anti-TNFSF13B antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: BAFF antibody can be used for detection of BAFF by Western blot a 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: BAFF antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF.
• Buffer: BAFF Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: BAFF antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of BAFF in human HL60 cell lysate (H) and mouse spleen tissue lysate (M) with BAFF antibody at 1 μg/mL. )

Immunocytochemistry (IHC)

(Immunocytochemistry of BAFF in HL60 cells with BAFF antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of BAFF in human spleen tissue with BAFF antibody at 20 μg/ml.Green: BAFF Antibody (2221)Blue: DAPI staining)

Related Product Information for anti-TNFSF13B antibody

BAFF Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-κB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.

NCBI and Uniprot Product Information

• NCBI GI #: 5730097
• NCBI GeneID: 10673
• NCBI Accession #: NP_006564
• NCBI GenBank Nucleotide #: NM_006573.4
• UniProt Accession #: Q9Y275; Q6FHD6; Q7Z5J2; E0ADT7
• Molecular Weight: 31 kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13B isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13b
• NCBI Official Symbol: TNFSF13B
• NCBI Official Synonym Symbols: DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13B; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13B
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF13B
• UniProt Synonym Gene Names: BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
• UniProt Entry Name: TN13B_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

BAFF: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Apoptosis; Membrane protein, integral; Cytokine

Chromosomal Location of Human Ortholog: 13q32-q34

Cellular Component: extracellular space; perinuclear region of cytoplasm; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; B cell costimulation; B cell homeostasis; immunoglobulin secretion; positive regulation of cell proliferation; T cell costimulation; positive regulation of B cell proliferation; positive regulation of germinal center formation; positive regulation of T cell proliferation; immune response; signal transduction