Western Blot (WB)
(Western Blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution.)
Rabbit BAF57 Polyclonal Antibody | anti-BAF57 antibody
BAF57 Polyclonal Antibody
Gene Names
SMARCE1; CSS5; BAF57
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
BAF57; Polyclonal Antibody; BAF57 Polyclonal Antibody; SMARCE1; BRG1 associated factor 57; BRG1-associated factor 57; Chromatin remodeling complex BRG1 associated factor 57; FLJ35648; SMARCE 1; SMCE1; SWI/SNF related matrix associated actin dependent regulator of chromatin e1; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; anti-BAF57 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BAF57 polyclonal antibody detects endogenous levels of BAF57 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
411
Applicable Applications for anti-BAF57 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2,000
ICC: 1:50-1:200
IF: 1:50-1:100
IHC: 1:50-1:200
IP: 1:50-1:200
ICC: 1:50-1:200
IF: 1:50-1:100
IHC: 1:50-1:200
IP: 1:50-1:200
Immunogen
Recombinant protein within human BAF57 aa 1-200.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-BAF57 antibody
The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF1 or SNF2alpha) and Brg-1 (also designated SNF2 or SNF2beta) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. In higher eukaryotes, BAF57 is also a critical component of the SWI/ SNF complex. BAF57 contains a high-mobility-group (HMG) domain adjacent to a kinesin-like region and is a DNA-binding subunit of the SWI/SNF complex. The human BAF57 gene maps within the q12-25 region of chromosome 17, a gene-rich area implicated in breast and ovarian cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47kDa
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
NCBI Official Symbol
SMARCE1
NCBI Official Synonym Symbols
CSS5; BAF57
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
UniProt Gene Name
SMARCE1
UniProt Synonym Gene Names
BAF57; BAF57
UniProt Entry Name
SMCE1_HUMAN
NCBI Description
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]
Research Articles on BAF57
Similar Products
Product Notes
The BAF57 smarce1 (Catalog #AAA3012288) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BAF57 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BAF57 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2,000 ICC: 1:50-1:200 IF: 1:50-1:100 IHC: 1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the BAF57 smarce1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAF57, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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