Western Blot (WB)
(Anti-BACE1C Antibody (Center) at 1:1000 dilution + human pancreas lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human BACE1C Polyclonal Antibody | anti-BACE1 antibody
BACE1C Antibody (Center)
IHC-P:~~1:50~100
FC:~~1:10~50
Western Blot (WB)
(Anti-BACE1C Antibody (Center) at 1:1000 dilution + human pancreas lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-BACE1C Antibody (Center) at 1:1000 dilution + human pancreas lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of anti-BACE1C Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). BACE1C(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-BACE1C Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). BACE1C(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H460 cells using BACE1C Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H460 cells using BACE1C Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Background: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]
Uniprot Description
BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.
Protein type: EC 3.4.23.46; Protease; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23.2-q23.3
Cellular Component: axon; cell surface; cytoplasmic vesicle membrane; endoplasmic reticulum lumen; endosome; endosome membrane; Golgi apparatus; integral to membrane; integral to plasma membrane; late endosome; multivesicular body; plasma membrane; trans-Golgi network
Molecular Function: aspartic-type endopeptidase activity; beta-amyloid binding; beta-aspartyl-peptidase activity; enzyme binding; peptidase activity; protein binding
Biological Process: beta-amyloid metabolic process; cellular protein metabolic process; membrane protein ectodomain proteolysis; protein catabolic process; proteolysis
Research Articles on BACE1
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Product Notes
The BACE1 bace1 (Catalog #AAA9217129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BACE1C Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BACE1C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA). WB:~~1:1000 IHC-P:~~1:50~100 FC:~~1:10~50. Researchers should empirically determine the suitability of the BACE1 bace1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACE1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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