Rabbit BACE1 Polyclonal Antibody | anti-BACE1 antibody

BACE1 Antibody

Cat. Num. AAA9613671

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: BACE1; Polyclonal Antibody; BACE1 Antibody; APP beta secretase; Asp 2; ASP2; Aspartyl protease 2; BACE 1; BACE; BACE1_HUMAN; Beta secretase 1; Beta secretase; Beta site amyloid beta A4 precursor protein cleaving enzyme; Beta site amyloid precursor protein cleaving enzyme 1; Beta site amyloid precursor protein cleaving enzyme; Beta site APP cleaving enzyme 1; Beta site APP cleaving enzyme; Beta-secretase 1; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; FLJ90568; HSPC104; Memapsin 2; Memapsin-2; Memapsin2; Membrane associated aspartic protease 2; Membrane-associated aspartic protease 2; Transmembrane aspartic proteinase Asp2; anti-BACE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: BACE1 Antibody detects endogenous levels of total BACE1.; Tissue Specificity: Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-BACE1 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human BACE1.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions (By similarity). Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein.Palmitoylation mediates lipid raft localization.Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8; inhibits lysosomal degradation.Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface.
• Subunit Structure: Monomer. Interacts (via DXXLL motif) with GGA1, GGA2 and GGA3 (via their VHS domain); the interaction highly increases when BACE1 is phosphorylated at Ser-498. Interacts with RTN3 and RTN4. Interacts with SNX6. Interacts with PCSK9. Interacts with NAT8 and NAT8B. Interacts with BIN1. Interacts (via extracellular domain) with ADAM10 (via extracellular domain) (By similarity). Interacts with SORL1; this interaction may affect binding with APP and hence reduce APP cleavage.
• Similarity: DXXLL motif is required for a proper endocytosis and retrograde transport to the trans-Golgi network, as well as for regulation of lysosomal degradation.The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.Belongs to the peptidase A1 family.
• Subcellular Location: Cell membrane>Single-pass type I membrane protein. Golgi apparatus>trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane>Single-pass type I membrane protein. Membrane raft. Lysosome. Late endosome. Early endosome. Recycling endosome. Cell projection>Axon. Cell projection>Dendrite.; Note: Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface (PubMed:17425515, PubMed:11466313). Colocalization with APP in early endosomes is due to addition of bisecting N-acetylglucosamine wich blocks targeting to late endosomes and lysosomes (By similarity). Retrogradly transported from endosomal compartments to the trans-Golgi network in a phosphorylation- and GGA1- dependent manner (PubMed:15886016).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-BACE1 antibody

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1 (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 333440466
• NCBI GeneID: 23621
• NCBI Accession #: NP_001193977.1
• NCBI GenBank Nucleotide #: NM_001207048.1
• UniProt Accession #: P56817; Q9BYB9; Q9BYC0; Q9BYC1; Q9UJT5; Q9ULS1; A0M8W7; B0YIU9; E9PE65; H7BXJ9
• Molecular Weight: 55,711 Da
• NCBI Official Full Name: beta-secretase 1 isoform E
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2
• UniProt Entry Name: BACE1_HUMAN

NCBI Description

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; EC 3.4.23.46; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis