Rabbit anti-Human B4GALT3 Polyclonal Antibody | anti-B4GALT3 antibody

B4GALT3 (Beta-1,4-galactosyltransferase 3, Beta-1,4-GalTase 3, Beta4Gal-T3, b4Gal-T3, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3, N-acetyllactosamine Synthase, Nal Synthase

Cat. Num. AAA6370870

• Gene Names: B4GALT3; beta4Gal-T3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: B4GALT3; Polyclonal Antibody; B4GALT3 (Beta-1; 4-galactosyltransferase 3; Beta-1; 4-GalTase 3; Beta4Gal-T3; b4Gal-T3; UDP-galactose:beta-N-acetylglucosamine beta-1; UDP-Gal:beta-GlcNAc beta-1; N-acetyllactosamine Synthase; Nal Synthase; anti-B4GALT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human B4GALT3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-B4GALT3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human B4GALT3, aa1-393 (NP_003770.1).
• Immunogen Sequence: MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDQGPTFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNSLTYQLLARELGPLYTNITADIGTDPRGPRAPSGPRYPPGSSQAFRQEMLQRRPPARPGPLSTANHTALRGSH
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(B4GALT3 rabbit polyclonal antibody. Western Blot analysis of B4GALT3 expression in human spleen.)

Western Blot (WB)

(Western Blot analysis of B4GALT3 expression in transfected 293T cell lineby B4GALT3 polyclonal antibody. Lane 1: B4GALT3 transfected lysate (43.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-B4GALT3 antibody

B4GALT3 is responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Product Categories/Family for anti-B4GALT3 antibody

Antibodies; Enzymes; Glycosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 4502349
• NCBI GeneID: 8703
• NCBI Accession #: NP_003770.1
• NCBI GenBank Nucleotide #: NM_003779.2
• UniProt Accession #: O60512; O60910; Q9BPZ4; Q9H8T2; D3DVG3
• Molecular Weight: 15,654 Da
• NCBI Official Full Name: beta-1,4-galactosyltransferase 3
• NCBI Official Synonym Full Names: beta-1,4-galactosyltransferase 3
• NCBI Official Symbol: B4GALT3
• NCBI Official Synonym Symbols: beta4Gal-T3
• NCBI Protein Information: beta-1,4-galactosyltransferase 3
• UniProt Protein Name: Beta-1,4-galactosyltransferase 3
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT3
• UniProt Synonym Gene Names: Beta-1,4-GalTase 3; Beta4Gal-T3; b4Gal-T3
• UniProt Entry Name: B4GT3_HUMAN

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

B4GALT3: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Belongs to the glycosyltransferase 7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.90; Glycan Metabolism - keratan sulfate biosynthesis; EC 2.4.1.38; Membrane protein, integral; Transferase; Glycan Metabolism - N-glycan biosynthesis

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: Golgi membrane; Golgi apparatus; cytoplasm; integral to membrane

Molecular Function: beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; N-acetyllactosamine synthase activity; metal ion binding; galactosyltransferase activity

Biological Process: keratan sulfate metabolic process; cellular protein metabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification