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Western Blot (WB) (Western blot analysis of B4GALNT2 using HeLa whole cell lysates)

Rabbit anti-Human B4GALNT2 Polyclonal Antibody | anti-B4GALNT2 antibody

B4GALNT2 Antibody

Gene Names
B4GALNT2; B4GALT; GALGT2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
B4GALNT2; Polyclonal Antibody; B4GALNT2 Antibody; 4 N-acetylgalactosaminyltransferase 2; 4-GalNAc transferase; 4-N-acetylgalactosaminyltransferase; B4GALT; B4GN2_HUMAN; Beta 1; 4 N-acetylgalactosaminyltransferase/betal; 4 GalNAcT/Sda-GalNAcT; Beta-1; beta-1; 4-N-acetyl-galactosaminyl transferase 2; beta-4-N-acetylgalactosaminyltransferase; CT-GalNAc transferase; GALGT2; Sd(a) beta-1; sda beta-1; UDP-GalNAc:Neu5Aca2-3Galb-R b1; UDP-GalNAc:Neu5Acalpha2-3Galbeta-R beta1; anti-B4GALNT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
B4GALNT2 antibody detects endogenous levels of total B4GALNT2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
506
Applicable Applications for anti-B4GALNT2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human B4GALNT2
Subcellular Location
Golgi Apparatus Membrane.
Tissue Specificity
Widely expressed. Highly expressed in colon and to a lesser extent in kidney, stomach, ileum and rectum.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of B4GALNT2 using HeLa whole cell lysates)

Western Blot (WB) (Western blot analysis of B4GALNT2 using HeLa whole cell lysates)
Related Product Information for anti-B4GALNT2 antibody
Function: Involved in the synthesis of the Sd(a) antigen (Sia-alpha2, 3-[GalNAc-beta1, 4]Gal-beta1, 4-GlcNAc), a carbohydrate determinant expressed on erythrocytes, the colonic mucosa and other tissues. Transfers a beta-1, 4-linked GalNAc to the galactose residue of an alpha-2, 3-sialylated chain.
Similarity: Belongs to the glycosyltransferase 2 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 63 kDa
Predicted: 64 kDa
NCBI Official Full Name
beta-1,4 N-acetylgalactosaminyltransferase 2 isoform b
NCBI Official Synonym Full Names
beta-1,4-N-acetyl-galactosaminyltransferase 2
NCBI Official Symbol
B4GALNT2
NCBI Official Synonym Symbols
B4GALT; GALGT2
NCBI Protein Information
beta-1,4 N-acetylgalactosaminyltransferase 2
UniProt Protein Name
Beta-1,4 N-acetylgalactosaminyltransferase 2
UniProt Gene Name
B4GALNT2
UniProt Synonym Gene Names
GALGT2

NCBI Description

B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen (Montiel et al., 2003 [PubMed 12678917]).[supplied by OMIM, Mar 2008]

Uniprot Description

Involved in the synthesis of the Sd(a) antigen (Sia-alpha2,3-[GalNAc-beta1,4]Gal-beta1,4-GlcNAc), a carbohydrate determinant expressed on erythrocytes, the colonic mucosa and other tissues. Transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain.

Research Articles on B4GALNT2

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Product Notes

The B4GALNT2 b4galnt2 (Catalog #AAA9609589) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B4GALNT2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B4GALNT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the B4GALNT2 b4galnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B4GALNT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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