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Western Blot (WB) (B3GNT1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the B3GNT1 antibody detected the B3GNT1 protein (arrow).)

Rabbit anti-Human B3GNT1 Polyclonal Antibody | anti-B3gnt1 antibody

B3GNT1, CT (B3GNT1, B3GNT6, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, Poly-N-acetyllactosamine extension enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1)

Gene Names
B3gnt1; iGAT; iGNT; B3gnt6; BETA3GNT1; 1500032M01Rik
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
B3GNT1; Polyclonal Antibody; CT (B3GNT1; B3GNT6; N-acetyllactosaminide beta-1; 3-N-acetylglucosaminyltransferase; I-beta-1; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 1); Anti -B3GNT1; anti-B3gnt1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-B3gnt1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
B3GNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-394 amino acids from the C-terminal region of human B3GNT1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(B3GNT1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the B3GNT1 antibody detected the B3GNT1 protein (arrow).)

Western Blot (WB) (B3GNT1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the B3GNT1 antibody detected the B3GNT1 protein (arrow).)

Immunohistochemistry (IHC)

(B3GNT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of B3GNT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (B3GNT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of B3GNT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-B3gnt1 antibody
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen.
Product Categories/Family for anti-B3gnt1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
47,384 Da
NCBI Official Full Name
B3gnt1 protein
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
NCBI Official Symbol
B3gnt1
NCBI Official Synonym Symbols
iGAT; iGNT; B3gnt6; BETA3GNT1; 1500032M01Rik
NCBI Protein Information
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; poly-N-acetyllactosamine extension enzyme; I-beta-1,3-N-acetylglucosaminyltransferase; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
UniProt Protein Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
UniProt Gene Name
B3gnt1
UniProt Synonym Gene Names
B3gnt6; iGnT
UniProt Entry Name
B3GN1_MOUSE

Uniprot Description

B4GAT1: Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans.

Protein type: Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.149; Transferase

Cellular Component: Golgi apparatus; membrane; integral to membrane

Molecular Function: glucuronosyltransferase activity; metal ion binding; alpha(1,6)-fucosyltransferase activity; oligosaccharyl transferase activity; acetylgalactosaminyltransferase activity; alpha(1,3)-fucosyltransferase activity; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity; glucosyltransferase activity; O antigen polymerase activity; alpha-1,2-mannosyltransferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; 1-phenanthrol glycosyltransferase activity; phenanthrol glycosyltransferase activity; glycolipid mannosyltransferase activity; galactosyltransferase activity; dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity; dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity; fucosyltransferase activity; lipopolysaccharide-1,6-galactosyltransferase activity; alpha-1,3-galactosyltransferase activity; alpha-1,2-galactosyltransferase activity; 9-phenanthrol UDP-glucuronosyltransferase activity; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; UDP-glucose:glycoprotein glucosyltransferase activity; 1,2-dihydroxy-phenanthrene glycosyltransferase activity; 9-phenanthrol glycosyltransferase activity; transferase activity; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; beta-1,4-mannosyltransferase activity; cellulose synthase activity; mannosyltransferase activity; alpha-1,3-mannosyltransferase activity; alpha-1,6-mannosyltransferase activity; inositol phosphoceramide synthase activity

Biological Process: axon guidance; protein amino acid O-linked mannosylation; protein amino acid glycosylation

Research Articles on B3gnt1

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Product Notes

The B3gnt1 b3gnt1 (Catalog #AAA643829) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B3GNT1, CT (B3GNT1, B3GNT6, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, Poly-N-acetyllactosamine extension enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B3GNT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the B3gnt1 b3gnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GNT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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