Western Blot (WB)
(Western blot analysis of B3GALT6 Antibody (C-term) in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) was detected using the purified Pab.)
Rabbit anti-Human B3GALT6 Polyclonal Antibody | anti-B3GALT6 antibody
B3GALT6, CT (B3GALT6, Beta-1,3-galactosyltransferase 6, GAG GalTII, Galactosyltransferase II, Galactosylxylosylprotein 3-beta-galactosyltransferase, UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western blot analysis of B3GALT6 Antibody (C-term) in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of B3GALT6 Antibody (C-term) in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(B3GALT6 Antibody (C-term) in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) ) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B3GALT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(B3GALT6 Antibody (C-term) in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) ) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B3GALT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(B3GALT6 Antibody (C-term) flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(B3GALT6 Antibody (C-term) flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The enzyme encoded by this intronless gene is a beta-1,3-galactosyltransferase found in the medial Golgi apparatus, where it catalyzes the transfer of galactose from UDP-galactose to substrates containing a terminal beta-linked galactose moiety. The encoded enzyme has a particular affinity for galactose-beta-1,4-xylose found in the linker region of glycosamines. This enzyme is required for glycosaminoglycan synthesis. [provided by RefSeq, Jun 2013]
Uniprot Description
B3GALT6: Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues. Belongs to the glycosyltransferase 31 family.
Protein type: EC 2.4.1.134; Membrane protein, integral; Transferase; Glycan Metabolism - chondroitin sulfate biosynthesis; Glycan Metabolism - heparan sulfate biosynthesis
Chromosomal Location of Human Ortholog: 1p36.33
Cellular Component: Golgi membrane; membrane; integral to membrane; Golgi medial cisterna
Molecular Function: UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity; galactosylxylosylprotein 3-beta-galactosyltransferase activity; UDP-galactosyltransferase activity
Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; heparan sulfate proteoglycan biosynthetic process; protein amino acid glycosylation; pathogenesis
Disease: Spondyloepimetaphyseal Dysplasia With Joint Laxity, Type 1, With Or Without Fractures; Ehlers-danlos Syndrome, Progeroid Type, 2
Research Articles on B3GALT6
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Product Notes
The B3GALT6 b3galt6 (Catalog #AAA6000050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B3GALT6, CT (B3GALT6, Beta-1,3-galactosyltransferase 6, GAG GalTII, Galactosyltransferase II, Galactosylxylosylprotein 3-beta-galactosyltransferase, UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B3GALT6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the B3GALT6 b3galt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GALT6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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