Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Axl antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Rabbit anti-Human Axl Polyclonal Antibody | anti-Axl antibody
Axl Rabbit pAb
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:100
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Axl antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Axl antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using Axl Rabbit pAb at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using Axl Rabbit pAb at dilution of 1:100 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of human colon carcinoma using Axl antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of human colon carcinoma using Axl antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunopreciptation (IP)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Axl antibody . Western blot was performed from the immunoprecipitate using AXL antibody at a dilition of 1:1000.)
Immunopreciptation (IP)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Axl antibody . Western blot was performed from the immunoprecipitate using AXL antibody at a dilition of 1:1000.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; Protein kinase, TK; TK group; Axl family
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; phosphatidylserine binding; protein heterodimerization activity; protein-tyrosine kinase activity; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding; myosin heavy chain binding
Biological Process: forebrain cell migration; negative regulation of lymphocyte activation; platelet activation; organ regeneration; peptidyl-tyrosine phosphorylation; cell maturation; neuron migration; vagina development; negative regulation of tumor necrosis factor production; signal transduction; phagocytosis; positive regulation of natural killer cell differentiation; erythrocyte homeostasis; positive regulation of protein kinase B signaling cascade; ovulation cycle; natural killer cell differentiation; negative regulation of interferon-gamma production; protein kinase B signaling cascade; cellular response to extracellular stimulus; innate immune response; blood vessel remodeling; spermatogenesis; negative regulation of neuron apoptosis; positive regulation of cytokine and chemokine mediated signaling pathway; inflammatory response; vascular endothelial growth factor receptor signaling pathway; apoptotic cell clearance; secretion by cell
Research Articles on Axl
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Product Notes
The Axl axl (Catalog #AAA9143083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Axl Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Axl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200 IP: 1:50-1:100. Researchers should empirically determine the suitability of the Axl axl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Axl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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